摘要
目的:探索12周跑台运动对db/db小鼠心肌代谢和胰岛素抵抗的作用,并分析PPARα和MG53在其中的作用。方法:db/db小鼠和m/m小鼠随机分配到DC(db/db对照)、DE(db/db运动)、MC(m/m对照)或ME(m/m运动)组,每组10只。MC和ME组小鼠维持安静的生活方式;ME和DE组小鼠进行为期12周的跑台运动。第1、2周,小鼠的运动速度、时间、频率分别为7.0~13.3 m/min、15~20 min、4次/周;13.3 m/min、30~45 min、6次/周。第3~12周,上述参数维持在13.3 m/min、1 h、6次/周。结果:1)4个实验组间心脏质量无显著差异。DC组ANF、BNF和α-MHC的mRNA表达显著低于MC组,β-MHC表达高于MC组;DE组ANF和α-MHC的mRNA表达显著高于DC组;2)DC组小鼠血清总胆固醇和甘油三脂含量显著高于MC组,DE组血清总胆固醇和甘油三酯含量显著低于DC组;3)DC组Cpt1b,Acadm,Acadvl,Acaa2和Pdk1的mRNA表达显著高于MC组,且在12周跑台运动后降低;4)DC组PPARα的蛋白表达及CD36、PGC-1β和Fabp3的mRNA表达显著高于MC组;DE组PPARα的蛋白表达及CD36的mRNA表达显著低于DC组;5)与MC组相比,DC组MG53的mRNA表达显著增加;与DC组相比,DE组MG53的mRNA表达显著降低。4个实验组间MG53的蛋白表达未见显著不同;6)DC组p-β-IR(Tyr1146)及p-AKT(Ser473)的蛋白表达显著低于MC组,p-IRS1(Ser1101)蛋白表达高于MC组;DE组p-β-IR(Tyr1146)及p-AKT(Ser473)的蛋白表达显著高于DC组,p-IRS1(Ser1101)的蛋白表达低于DC组。结论:12周跑台训练显著改善db/db小鼠胰岛素抵抗状态和心肌代谢紊乱,且PPARα信号通路参与上述过程。
Objective:This study is to investigate the effect of 12-week treadmill running on myocardial metabolism and insulin resistance of db/db mice,as well as to explore the role of PPARαand MG53.Methods:db/db mice and m/m mice were randomly assigned to DC group(db/db control),DE group(db/db exercise),MC group(m/m control)or ME group(m/m exercise),with 10 mice in each group.The mice of MC group and DC group remained sedentary lifestyle,while the mice of ME group and DE group were trained to run on the treadmill for 12 weeks.For the first and second week,the running velocity,duration and frequency of the mice were 7.0 to 13.3 m/min,15 to 20 min,4 times once a week and 13.3 m/min,30 to 45 min and 6 times once a week.From the third week to the twelfth week,the above parameters were maintained at 13.3 m/min,1 h and 6 times once a week.Results:1)The heart weights were not significantly different among the four groups.The mRNA expressions of ANF,BNF andα-MHC were significantly lower in DC group than that in MC group.The expression ofβ-MHC was significantly higher in DC group than that in MC group.The mRNA expressions of ANF andα-MHC were significantly higher in DE group than that in DC group.2)The serum levels of total cholesterol and triglycerides were significantly higher in DC group than that in MC group,and the serum levels of total cholesterol and triglycerides were lower in DE group than that in DC group.3)The mRNA expressions of Cpt1b,Acadm,Acadvl,Acaa2 and Pdk1 were significantly higher in DC group than MC group,and those index were decreased after 12-week treadmill running.4)The protein expression of PPARαand the mRNA expressions of CD36,PGC-1βand Fabp3 were significantly higher in DC group than that in MC group.The protein expression of PPARαand the mRNA expression of CD36 were significantly lower in DE group than that in DC group.5)Compared to MC group,the mRNA expression of MG53 was significantly increased in DC group.Compared to DC group,the mRNA expression of MG53 was significantly decreased in DE group.T
作者
孙易
张怡
丁树哲
SUN Yi;ZHANG Yi;DING Shuzhe(Key Laboratory of Adolescent Health Assessment and Exercise Intervention of Ministry of Education,East China Normal University,Shanghai 200241,China;College of Physical Education and Health,East China Normal University,Shanghai 200241,China)
出处
《中国体育科技》
北大核心
2023年第4期67-76,共10页
China Sport Science and Technology
基金
国家自然科学基金项目(31600967)
中央高校基本科研业务费专项资金
青少年健康评价与运动干预教育部重点实验室建设项目(11000-30190-513300/006)。
