摘要
浮游细菌调控是对虾养殖水体环境控制策略的核心内容,探究浮游细菌群落构建的一般规律,可进一步推动对虾养殖水体水质调控技术的研究。运用16S rRNA高通量测序技术,对凡纳滨对虾(Litopenaeus vannamei)淡化养殖池塘进行了12次周际调查。结果显示,48个样品共获得2854个操作分类单元(Operational taxonomic units,OTU,97%相似性),序列比对发现古细菌2门1纲1科1属,细菌30门59纲98目199科433属,其中优势菌群25属。优势菌群在组成上有较高的相似性,但各池优势菌群在分布和相对丰度变动上有较大差异。各池系统发育多样性指数总平均值为77.57,变幅为24.39~111.65;香农多样性指数总平均值为3.96,变幅为2.64~5.06;物种丰富度指数总平均值为716,变幅为229~1054。非度量多维标度分析(Non-metric multidimensional scaling,NMDS)表明各池塘浮游细菌群落在养殖初期差异较大,中、后期差异减小,冗余分析(Redundancy analysis,RDA)显示活性磷、碱度、溶解氧和硫化物可显著影响浮游细菌的群落结构。
The regulation of bacterioplankton is the core content of the environmental control strategy in shrimp aquaculture.Exploring the general rules of the construction of bacterioplankton community can further promote the research on water quality regulation for shrimp ponds.Using 16s rRNA high-throughput sequencing technology,we conducted 12 weekly surveys in Litopenaeus vannamei desalinated ponds.The results indicate that a total of 2854 OTUs(97%similarity)were obtained from 48 samples(Archaea belonged to 2 phyla,1 class,1 family and 1 genus;and bacteria belonged to 30 phyla,59 classes,98 orders,199 families and 433 genera,among which 25 genera were dominant flora).The dominant flora had high similarity in the composition,but with great differences in the distribution and relative abundance in each pond.The total average phylogenetic diversity index was 77.57,ranging from 24.39 to 111.65;the total average Shannon diversity index was 3.96,ranging from 2.64 to 5.06;the total average species richness index was 716,ranging from 229 to 1054.NMDS analysis shows that the community structure of bacterioplankton in each pond varied greatly at the early stage of aquaculture,but not so greatly at the middle and late stages.The results of redundancy analysis shows that the labile phosphorus,total alkalinity,dissolved oxygen and sulfide were the main environmental factors affecting the distribution characteristics of community structure of bacterioplankton.
作者
祁峰
热比古丽·沙吾提
咸玉兰
韩军军
米乃瓦尔·木衣提
陈韩飞
孙世萍
马燕武
陈朋
QI Feng;SHAWUTI Rebiguli;XIAN Yulan;HAN Junjun;MUYITI Minawaer;CHEN Hanfei;SUN Shiping;MA Yanwu;CHEN Peng(Institute of Fishery Research of Xinjiang Uygur Autonenous Region,Urumqi 830099,China;Scientific Observing and Experimental Station of Fishery Resources and Environment in Northwest China,Ministry of Agriculture and Rural Affairs,Urumqi 830099,China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2023年第2期50-61,共12页
South China Fisheries Science
基金
新疆维吾尔自治区自然科学计划(自然科学基金)面上项目(2020D01A102)。
关键词
凡纳滨对虾池塘
浮游细菌
群落多样性
群落结构
理化因子
Litopenaeus vannamei pond
Bacterioplankton
Community diversity
Community structure
Phycicochemical factor
