摘要
目的 探究乙酰胆碱酯酶(acetylcholinesterase,Ache)、P-选择素(P-selectin)、细胞周期素依赖蛋白激酶5(cyclin-dependent kinase 5,CDK5)在病毒性脑炎(viral encephalitis,VE)中的表达及相关性。方法 本研究选取在本院健康体检及神经内科就诊治疗、住院治疗的病毒性脑炎患者130例作为研究对象,并纳入研究组,根据患者的病情时间将其分为急性期和恢复期,其中急性期组85例,恢复期组45例。根据患者的临床表现,将其分为轻度组和重度组,其中轻度组76例,重度组54例,选择同期在我院接受体检的健康患者60例作为对照组。应用丁酰硫代胆碱底物法检测Ache水平;酶联免疫吸附法检测血清P-selectin水平;CDK5分离试剂盒检测CDK5水平。观察研究组和对照组、急性期和恢复期及重度组和轻度组患者Ache、P-selectin、CDK5水平,分析病毒性脑炎患者Ache、P-selectin和CDK5之间相关性,利用ROC曲线比较Ache、P-selectin、CDK5单独检测及联合检测在VE中的诊断价值。结果 研究组Ache、P-selectin、CDK5水平显著高于对照组(P<0.05);急性期患者Ache、P-selectin、CDK5水平显著高于恢复期(P<0.05);重度组患者Ache、P-selectin、CDK5水平显著高于轻度组(P<0.05);Ache、P-selectin和CDK5蛋白表达两两之间均呈正相关(P<0.05);联合诊断的诊断价值显著高于Ache、P-selectin、CDK5单独诊断(Z=2.765、2.563、2.925,P均<0.05)。结论 Ache、P-selectin、CDK5均参与病毒性脑炎患者发病过程,在发病不同时期呈现不同水平表达,且两两指标之间呈现正相关,可以作为病毒性脑炎患者的诊断和治疗依据。
Objective To explore the expression of acetylcholinesterase(Ache),P-selectin,and cyclin-dependent kinase 5(CDK5)in viral encephalitis(VE)and their correlation.Methods A total of 130 patients with viral encephalitis who underwent physical examination,presented to Department of Neurology or treated in our hospital were selected as the research subjects,and included in the research group.The patients were divided into acute phase group(n=85)and recovery phase(n=45)according to severity of their illness.According to the clinical manifestations of patients,they were divided into mild group(n=76)and severe group(n=54).Sixty healthy people who underwent physical examination in our hospital during the same period were used as the control group.The butyrylthiocholine substrate method was used to detect the level of Ache,the enzyme-linked immunosorbent method was used to detect the level of serum P-selectin,and the CDK5 separation kit was used to detect the level of CDK5.The Ache,P-selectin,and CDK5 levels of patients in the research group and control group,acute phase and recovery phase,and severe and mild groups were observed.The correlation between Ache,P-selectin and CDK5 in patients with VE was analyzed.The ROC curve was used to compare the diagnostic value of detection of Ache,P-selectin,and CDK5 alone or in combination in VE.Results The levels of Ache,P-selectin and CDK5 were significantly higher in the research group than in the control group(P<0.05),higher in the acute phase than in the recovery phase(P<0.05),and higher in the severe group than in the mild group(P<0.05).Ache,P-selectin and CDK5 protein expressions were positively correlated(P<0.05).The diagnostic value of combined diagnosis was significantly higher than that of Ache,P-selectin,and CDK5 alone(Z=2.765,2.563,2.925,all P<0.05).Conclusion Ache,P-selectin and CDK5 are all involved in the pathogenesis of VE patients.They are expressed at different levels at different stages of the onset,and there is a positive correlation between each two indicators.Ther
作者
贺龙
王莎
王静
李素婷
霍瑞民
HE Long;WANG Sha;WANG Jing;LI Su-ting;HUO Rui-min(The Third Department of Neurology,the First Hospital of Handan City,Hebei Province,Handan 056004,China;The Second Department of Neurology,the First Hospital of Handan City,Hebei Province,Handan 056002,China)
出处
《河北医科大学学报》
CAS
2023年第4期392-396,411,共6页
Journal of Hebei Medical University
基金
河北省医学科学研究课题计划(20200439)。
