摘要
通过DNAstar和IEDB等生物信息学软件对7种芝麻过敏原的生物学信息进行分析,采用多种方案对芝麻过敏原的抗原指数、亲水性、表面可及性、柔韧性等参数及其二级结构进行预测,并对预测的抗原表位综合分析.利用SWISS-MODEL进行同源建模并用拉氏图评价其结构稳定性.结果表明,7种芝麻过敏原蛋白均存在多个可能的抗原表位,采用同源建模的方式成功构建了芝麻过敏原蛋白的三级结构模型,拉氏图显示该模型的构象稳定.本研究为制备特异性抗体肽段和过敏原检测等提供了依据.
In this study the biological information of seven sesame allergens was analyzed via bioinformatics software such as DNAstar and IEDB.The antigenic index,hydrophilicity,surface accessibility,flexibility and secondary structure of sesame allergens were predicted using(or with)various approaches(or methods).A comprehensive analysis of the predicted antigenic epitopes was performed.SWISS-MODEL was used for homology modeling and Ramachandran Plot was used to evaluate structural stability.The results showed that there were multiple possible epitopes in the seven sesame allergen proteins.The tertiary structure model of the sesame allergen protein was successfully constructed by homology modeling,and the conformational stability of the model was evaluated by Ramachandran Plot.The results could be useful for the preparation of specific antibody peptides and allergens detection.
作者
令狐晓盼
王莎莎
邱金平
陆旸
LINGHU Xiaopan;WANG Shasha;QIU Jinping;LU Yang(College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China)
出处
《天津科技大学学报》
CAS
2023年第2期19-27,74,共10页
Journal of Tianjin University of Science & Technology
