摘要
为明确洛阳烟区烤烟品系LY1306上六片适宜的一次性采收成熟度,采用田间试验,设置4种采收成熟度处理(M1、M2、M3和M4),研究其对上六片3个叶位(上_(1~2)片叶、上_(3~4)片叶和上_(5~6)片叶)叶绿素相对含量(SPAD值)、叶色相对值、丙二醛(MDA)含量、抗氧化酶活性及碳、氮代谢关键酶活性的影响,并分析烤后烟叶常规化学成分和感官质量的差异。结果表明,随着采收成熟度的提高,上六片3个叶位的超氧化物歧化酶与淀粉酶活性均表现为先升高后下降的变化趋势,在M3处理期达到最大值;过氧化物酶、过氧化氢酶和硝酸还原酶活性逐渐下降,MDA含量逐渐升高,且M3到M4处理阶段变化幅度加大;SPAD值与叶色相对值随着采收成熟度的提高而降低,至M3和M4处理期时,上六片3个叶位的SPAD值均差异不显著,且叶色相对值也趋于稳定;M3处理烤后烟叶的总糖、还原糖含量以及糖碱比均最高,焦甜香味突出,评吸总分最高。总之,建议LY1306在洛阳烟区以M3处理期(上_(3~4)叶叶面落黄7~8成、主脉全白、支脉2/3变白,叶面皱褶,SPAD值为18左右,叶色相对值为1.07左右)一次性采烤上六片为宜。
In order to determine the suitable one-time harvest maturity of upper six leaves of flue-cured tobacco line LY1306 in Luoyang tobacco region,four harvest maturity treatments(M1,M2,M3 and M4)were set up in field,and the effects of different harvest maturity on the chlorophyll content(SPAD value),leaf color relative value,malondialdehyde(MDA)content,antioxidant enzyme activities,and key enzyme activities of carbon and nitrogen metabolism at three leaf positions(upper_(1~2) leaves,upper_(3~4) leaves and upper_(5~6) leaves)of upper six leaves were investigated,and the differences of the conventional chemical composition and sensory quality in cured tobacco leaves were further analyzed.The results showed that,the activities of superoxide dismutase and amylase increased first and then decreased with the increase of harvest maturity,and reached the maximum at M3 treatment stage.The activities of peroxidase,catalase and nitrate reductase decreased gradually,the content of MDA increased gradually,and the change range of M3 to M4 treatment stages were further increased compared with other treatment stages.SPAD value and leaf color relative value decreased with the increase of harvest maturity,there was no significant difference in SPAD values among three leaf positions,and the leaf color relative value also tended to be stable at M3 and M4 treatment stages.The total sugar,reducing sugar content and sugar-alkali ratio of flue-cured tobacco leaves were all the highest,the burned-sugar aroma was prominent and the total score of sensory evaluation was the highest in M3 treatment.Taken together,the upper six leaves of line LY1306 was suitable for one-time harvest at M3 treatment stage(when upper_(3~4) leaves were 70%~80%yellow,the main veins became all white and the branch veins became 2/3 white,the leaf surface turned wrinkled,the SPAD value was about 18,as well as the leaf color relative value was about 1.07)in Luoyang tobacco region.
作者
王悦华
周俊学
马宜林
马君红
王艳芳
赵世民
申洪涛
李友军
刘领
Wang Yuehua;Zhou Junxue;Ma Yilin;Ma Junhong;Wang Yanfang;Zhao Shimin;Shen Hongtao;Li Youjun;Liu Ling(College of Agriculture,Henan University of Science and Technology,Luoyang 471023,Henan,China;Technology Center,Luoyang Branch of Henan Provincial Tobacco Corporation,Luoyang 471000,Henan,China;Technology Center,China Tobacco Henan Industrial Limited Company,Zhengzhou 450016,Henan,China)
出处
《作物杂志》
北大核心
2023年第2期171-177,共7页
Crops
基金
河南省重点研发与推广专项(科技攻关)(212102110286)
河南省烟草公司洛阳市公司项目(LYKJ202003,2022410300270071)
河南中烟工业有限责任公司资助项目(2020410001340006)。
关键词
烤烟
品系LY1306
上六片
采收成熟度
生理代谢
品质
Flue-cured tobacco
Line LY1306
Upper six leaves
Harvest maturity
Physiological metabolism
Quality
