摘要
目的通过一种步骤简单、成本低的方法制备新型Mn_(3)O_(4)纳米簇,其可做为T1磁共振对比剂,并进一步探索其在体内外的造影性能以及生物安全性。方法利用透射电子显微(TEM)观察Mn_(3)O_(4)纳米材料的形貌,通过动态光散射仪(DLS)检测纳米材料的水合粒径和Zeta电位,借助傅里叶变换红外光谱仪(FT-IR)、X射线衍射仪(XRD)和比表面积与孔隙度分析仪测试Mn_(3)O_(4)纳米簇的组分、晶体结构和比表面积,通过3.0 T磁共振扫描仪分析Mn_(3)O_(4)纳米簇的磁共振造影性能,并利用MTT实验和溶血实验评价纳米簇的生物安全性。结果采用水热法成功制备Mn_(3)O_(4)纳米簇,其粒径大约为100 nm。Mn_(3)O_(4)纳米簇展示较高的比表面积和丰富的介孔结构,具有良好的单分散性和胶体稳定性。此外,Mn_(3)O_(4)纳米簇在弱酸环境下能够释放出大量的锰离子,其游离的锰离子可作为T1磁共振对比剂,提高病灶组织与正常组织的对比度。在pH值为4.5的条件下,Mn_(3)O_(4)纳米簇的纵向弛豫率(r1)值为6.1485 mM^(-1)·s^(-1),其在体内外的成像均展现出良好的T1磁共振造影性能。此外,Mn_(3)O_(4)纳米簇几乎不影响正常细胞THLE-3的相对存活率,同时溶血实验结果也表明Mn_(3)O_(4)纳米簇不会损伤红细胞,证明Mn_(3)O_(4)纳米簇具有良好的生物相容性。结论具有pH响应能力的Mn_(3)O_(4)纳米簇展示出优异的T1磁共振成像性能和高的生物相容性,具有广泛的临床应用前景。
Objective To prepare novel Mn_(3)O_(4)nanoclusters as a T1 MR contrast agent by a simple and low-cost method,and to explore its contrast performance in vitro and in vivo and biosafety.Methods Transmission electron microscopy(TEM)was used to observe the morphology of Mn_(3)O_(4)nanoclusters,a dynamic laser scattering(DLS)detector was used to detect the hydronamic size and zeta potential of Mn_(3)O_(4)nanoclusters,the components,crystal structure and specific surface area of Mn_(3)O_(4)nanomaterials were respectively explored by fourier transform infrared(FT-IR)spectrometer,X-ray diffraction(XRD)and accelerated surface area and porosimetry system,the magnetic resonance contrast performance of Mn_(3)O_(4)nanoclusters was analyzed by 3.0 T MRI scanner and the cell biological safety of nanoclusters was evaluated by MTT experiment and hemolysis experiment.Results Mn_(3)O_(4)nanoclusters with clustered structure were successfully prepared by hydrothermal method,and the diameter of nanoclusters was about 100 nm.Mn_(3)O_(4)nanoclusters have a large specific surface area and abundant mesoporous structure,while it has good monodispersity and colloidal stability.Massive manganese ions were released from Mn_(3)O_(4)nanoclusters under weak acid environment,and it could serve as T1 MR contrast agents for improving the contrast between normal and lesion tissue.The longitudinal relaxation rate(r1)value of Mn_(3)O_(4)nanoclusters were 6.1485 mM^(-1)·s^(-1)in pH 4.5 solution,implying nanoclusters possessed the good T1 contrast performance.In addition,Mn_(3)O_(4)nanoclusters hardly affected the relative cell viability of THLE-3 cells,and the data about hemolysis rate further demonstrated that nanoclusters couldn′t damage red cells,indicating that Mn_(3)O_(4)nanocluster possessed excellent biocompatibility.Conclusion Mn_(3)O_(4)nanoclusters with pH response demonstrated excellent T1 contrast performance and high biocompatibility,showing good application prospects.
作者
王鹏
王银凤
肖建敏
田梗
张桂龙
WANG Peng;WANG Yinfeng;XIAO Jianmin;TIAN Geng;ZHANG Guilong(College of Pharmacy,Binzhou Medical University,Yantai 264003,Shandong,P.R.China;Institute of Medical Imaging Probe,Binzhou Medical University,Yantai 264003,Shandong,P.R.China)
出处
《滨州医学院学报》
2023年第2期133-139,共7页
Journal of Binzhou Medical University
基金
国家自然科学基金青年基金(22007006)
山东省泰山学者青年专家(tsqn201909144)
滨州医学院高层次人才科研启动基金(BY2019KYQD46)。
