摘要
通过探究在不同胃肠道胁迫条件下,药食同源中药材滇黄精总多糖和水提物对罗伊氏乳杆菌1.2838群体感应信号分子自诱导物-2(autoinducers-2,AI-2)的影响,分析其对罗伊氏乳杆菌1.2838的保护作用。将活化的罗伊氏乳杆菌1.2838与滇黄精总多糖和水提物分别进行共孵育至其OD600 nm为0.6~0.8,将处理后的益生菌在胃肠道胁迫条件下培养1 h~3 h后检测AI-2的生成量。结果表明,滇黄精总多糖和水提物在一定程度上能提高罗伊氏乳杆菌1.2838在胃肠道胁迫条件下AI-2的生成量,滇黄精水提物对罗伊氏乳杆菌1.2838的保护作用优于滇黄精总多糖。
Inorder to investigate the protective effect of the Polygonatum kingianum polysaccharide and water extracts on Lactobacillus reuteri 1.2838 under different gastrointestinal stress conditions,the content of quorum-sensing molecules autoinducers-2(AI-2)was measured.The activated L.reuteri 1.2838 was co-incubated with either the P.kingianum polysaccharide or water extract until OD600 nm reached 0.6-0.8.The treated probiotics were cultured for 1 h-3 h under gastrointestinal stress conditions to detect the production of AI-2.The results showed that the P.kingianum polysaccharide and water extracts could partially increase the production of L.reuteri 1.2838 AI-2 under different gastrointestinal stress conditions.However,the water extract exhibited a better protective effect on L.reuteri 1.2838 than that shown by the polysaccharide extract.
作者
陈秋定
孟凡颖
谢海娜
张祥
王文博
杨兴鑫
张范
李静平
顾雯
贺森
CHEN Qiu-ding;MENG Fan-ying;XIE Hai-na;ZHANG Xiang;WANG Wen-bo;YANG Xing-xin;ZHANG Fan;LI Jing-ping;GU Wen;HE Sen(School of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,Yunnan,China)
出处
《食品研究与开发》
CAS
北大核心
2023年第8期201-206,共6页
Food Research and Development
基金
云南省基础研究计划项目中医联合专项-面上项目(202001AZ070001-037)
昆明市国际(对外)科技合作基地项目(GHJD-2021030)
云南省基础研究计划-面上项目(202001AT070109)
云南省“兴滇英才支持计划”青年人才项目(XDYC-QNRC-2022-0469)
云南省“万人计划”青年拔尖人才专项(YNWR-QNBJ-2020-279)。
