摘要
本试验以藜麦品种‘Tomico quinoa’作为研究材料,缓解剂硝普钠(SNP)作为外源NO(Nitric oxide)供体,研究不同浓度梯度SNP(100,200,400μmol/L)处理对150 mmol/L NaCl胁迫下藜麦幼苗形态及生理特性的影响。本研究测定藜麦幼苗的株高(PH)、鲜重(FW)、叶绿素(Chl)、可溶性糖(SS)、脯氨酸(Pro)、可溶性蛋白(SP)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、丙二醛(MDA),并分析各指标与藜麦幼苗耐盐能力之间的关系。结果表明,150 mmol/L Na Cl明显抑制了藜麦幼苗的生长,而SNP处理则能一定程度上缓解NaCl胁迫对藜麦幼苗造成的伤害。其中SNP处理显著提高了盐胁迫下藜麦幼苗叶光合色素含量以及渗透调节物质含量的积累,同时增强了抗氧化酶的活性,且显著降低了膜脂过氧化产物的含量,使其恢复到正常水平。由此推测外源NO在缓解藜麦幼苗盐胁迫中起到了积极的作用。
In order to study the influence in morphology and physiology of quinoa seedlings under 150 mmol/L NaCl stress and SNP(100,200,400μmol/L)treatments,the variety'Tomico Quinoa'were used as materials and alleviator sodium nitroprusside(SNP)was used as a donor for exogenous NO(Nitric oxide).This study deter-mined plant height(PH),fresh weight(FW),chlorophyll(Chl),soluble sugar(SS),proline(Pro),soluble protein(SP),superoxide dismutase(SOD),peroxidase(POD),Malondialdehyde(MDA),and also analyzes the relationship between each index and the salt tolerance of quinoa seedlings.The results showed that the growth of quinoa seedlings was inhibited under 150 mmol/L NaCl obiviously,while SNP treatments could alleviate the damage caused by NaCl stress on seedlings to a certain extent.The photosynthetic pigment contents and the accumulation of osmotic regulators in quinoa seedlings have been increased significantly under salt stress by using SNP.Also,it enhanced the activity of antioxidant enzymes,and reduced the contents of membrane lipid peroxidation products significantly so that they returned to a normal level.It is speculated that exogenous NO plays an active role in alleviating the salt stress of quinoa seedlings.
作者
吴铮
钱锋
姜嵩
袁俊杰
蒋玉蓉
陆国权
Wu Zheng;Qian Feng;Jiang Song;Yuan Junjie;Jiang Yurong;Lu Guoquan(College of Agriculture and Food Sciences,Zhejiang A&F University,Hangzhou,311300;College of Chemistry and Life Science,Zhejiang Normal University,Jinhua,321004)
出处
《分子植物育种》
CAS
北大核心
2023年第7期2344-2349,共6页
Molecular Plant Breeding
基金
国家自然科学基金项目(31301372)
浙江省重大科技专项(2011C12030)
青海省海西州科技项目(2012-Y01)共同资助。
关键词
藜麦
NO
盐胁迫
渗透调节
抗氧化酶
Quinoa
NO
Salt stress
Osmotic adjustment
Antioxidase
