摘要
目的:探究膀胱癌组织钙调蛋白结合蛋白(CALD1)信使RNA(mRNA)表达水平与临床病理特征和预后的关系。方法:选取2015年7月-2017年7月于我院收治并确诊的111例膀胱癌患者为研究对象,收集癌组织及癌旁正常组织,采用逆转录聚合酶链式反应(RT-PCR)法检测CALD1 mRNA在膀胱癌组织与癌旁正常组织中的表达差异,分析CALD1 mRNA相对表达量与临床病理特征的关系。对患者进行5年随访,分析CALD1 mRNA相对表达量与预后的关系。结果:CALD1 mRNA在膀胱癌组织的相对表达量显著高于癌旁正常组织(P<0.05);CALD1 mRNA相对表达量与膀胱癌患者组织分化程度、TNM分期、淋巴结转移相关(P<0.05);111例患者出院后随访5年,4例失访,107例患者获得随访,CALD1低表达组和高表达组的5年总生存率分别为72.34%(34/47)、50.00%(30/60)(P<0.05),CALD1低表达组5年总生存率高于高表达组;Cox比例风险回归模型分析显示,组织低分化程度、TNM高分期、淋巴结转移、CALD1 mRNA高表达是影响膀胱癌患者预后的危险因素(P<0.05)。结论:CALD1在膀胱癌组织中呈高表达,CALD1 mRNA相对表达量与患者组织分化程度、TNM分期、淋巴结转移及预后相关。
Objective: To investigate the relationship between the calmodulin binding protein(CALD1) messenger RNA(mRNA) expression level and clinicopathological features and prognosis in bladder cancer tissue. Methods: 111 patients with bladder cancer who were admitted and diagnosed in our hospital from July 2015 to July 2017 were selected as the research objects. The cancer tissues and normal tissues adjacent to cancer were collected. The expression difference of CALD1 mRNA in bladder cancer tissues and normal tissues adjacent to the cancer was detected by reverse transcription polymerase chain reaction(RT-PCR), and the relationship between the relative expression of CALD1 mRNA and clinicopathological features was analyzed. The patients were followed up for 5 years, the relationship between the relative expression of CALD1 mRNA and prognosis were analyzed. Results: The relative expression of CALD1 mRNA in bladder cancer was significantly higher than that in normal tissues adjacent to cancer(P<0.05). The relative expression of CALD1mRNA was correlated with degree of tissue differentiation, TNM stage and lymph node metastasis of patients with bladder cancer(P<0.05). 111 patients were followed up for 5 years after discharge, 4 were lost to follow-up, and 107 patients were followed up. The5-year overall survival rate of low CALD1 expression group and high CALD1 expression group were 72.34%(34/47) and 50.00%(30/60), respectively(P<0.05). The 5-year overall survival rate of low CALD1 expression group was higher than that of high CALD1 expression group. Cox proportional hazards regression analysis showed that low degree of tissue differentiation, high TNM stage, lymph node metastasis and high CALD1 mRNA expression were the risk factors affecting the prognosis of patients with bladder cancer(P<0.05).Conclusion: CALD1 is highly expressed in bladder cancer tissue, and the relative expression of CALD1 mRNA is related to the degree of tissue differentiation, TNM stage, lymph node metastasis and prognosis.
作者
熊文
尚光宇
向波
杨博
麻慧
XIONG Wen;SHANG Guang-yu;XIANG Bo;YANG Bo;MA Hui(Department of Urology Surgery,The First Affiliated Hospital of Jishou University,Jishou,Hunan,416000,China;Department of Clinical Laboratory,The First Affiliated Hospital of Jishou University,Jishou,Hunan,416000,China;Department of Pathology,The First Affiliated Hospital of Jishou University,Jishou,Hunan,416000,China)
出处
《现代生物医学进展》
CAS
2023年第3期529-533,共5页
Progress in Modern Biomedicine
基金
湖南省卫计委科研基金项目(C2015-83)
吉首大学校级科研项目资助(Jdzd21007)。
