摘要
爪哇镰刀菌和尖孢镰刀菌对甘薯具有强致病力,为了筛选对2种病原菌防效较好的药剂,采用菌丝生长速率法分别测定多菌灵、甲基硫菌灵、腐酶利、肟菌·戊唑醇、唑醚·代森联、咪鲜胺、中生菌素和四霉素对2株甘薯致病镰刀菌的室内毒力。结果表明,对爪哇镰刀菌菌株菌丝生长抑制作用最强的为四霉素和咪鲜胺,EC50分别为1.292、2.145μg/mL,其次为中生菌素和肟菌·戊唑醇,EC50分别为16.652、28.641μg/mL,腐酶利、甲基硫菌灵、多菌灵和唑醚·代森联对爪哇镰刀菌菌株的抑制作用最差,EC50分别为440.540、637.120、680.765μg/mL和22385.367μg/mL;对尖孢镰刀菌菌株菌丝生长抑制作用最强的为四霉素、咪鲜胺和肟菌·戊唑醇,EC50分别为0.03、0.131μg/mL和0.177μg/mL,其次为中生菌素和多菌灵,EC50分别为3.66、14.408μg/mL。甲基硫菌灵和唑醚·代森联对尖孢镰刀菌菌株菌丝生长抑制作用较差,EC50分别为28.611、59.78μg/mL,腐酶利抑制作用最差,EC50为379.426μg/mL。四霉素和咪鲜胺对2种病原菌的生长均有明显的抑制作用,2种病原菌对不同药剂的敏感性存在显著差异,尖孢镰刀菌对8种药剂的敏感性明显高于爪哇镰刀菌。
Fusarium solani-melongenae and Fusarium oxysporum have strong pathogenicity to sweet patato.To screen effective fungicides to control them,the indoor toxicity of eight fungicides to the two strains of Fusarium spp.pathogens were detected by using mycelial growth rate method,and the eight fungicides were carbendazim,thiophanate-methyl,procymidone,trifloxystrobin·tebuconazole,pyraclostrobin·metiram,prochloraz,zhongshengmycin and tetramycin.The results showed that tetramycin and prochloraz were found to be the most effective fungicides inhibiting the mycelial growth of Fusarium solani-melongenae,and the mean effective concentration(EC50)value was 1.292μg/mL and 2.145μg/mL,respectively.The EC50 value of zhongshengmycin and trifloxystrobin·tebuconazole was 16.652μg/mL and 28.641μg/mL respectively,also effective against Fusarium solani-melongenae.However,procymidone,thiophanate-methyl,carbendazim and pyraclostrobin·metiram,with EC50 value of 440.540,637.120,680.765 and 22385.367μg/mL respectively,were less effective against Fusarium solani-melongenae.Tetramycin,prochloraz and trifloxystrobin·tebuconazole were the most effective fungicides inhibiting the mycelial growth of Fusarium oxysporum,and the mean effective concentration(EC50)value was 0.03,0.131 and 0.177μg/mL,respectively.Zhongshengmycin and carbendazim were also effective against Fusarium oxysporum,and the EC50 value was 3.66μg/mL and 14.408μg/mL,respectively.Thiophanate-methyl and pyraclostrobin·metiram had poor inhibitory effect on the mycelial growth of Fusarium oxysporum strains,with EC50 of 28.611μg/mL and 59.78μg/mL,respectively.The inhibitory effect of procymidone was the worst,its EC50 value was 379.426μg/mL.Overall,tetramycin and prochloraz could obviously inhibit the two strains of Fusarium spp.,and there were significant differences of the sensitivity of the two strains of Fusarium spp.to different fungicides.The sensitivity of Fusarium oxysporum to the eight fungicides was higher than that of Fusarium solani-melongenae.
作者
陈新亮
陈景益
罗忠霞
王章英
房伯平
王小斌
黄立飞
CHEN Xinliang;CHEN Jingyi;LUO Zhongxia;WANG Zhangying;FANG Boping;WANG Xiaobin;HUANG Lifei(Crop Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Crops Genetics and Improvement,Guangzhou 510640)
出处
《中国农学通报》
2023年第8期106-111,共6页
Chinese Agricultural Science Bulletin
基金
财政部和农业农村部国家现代农业产业技术体系资助(CARS-10-C17,CARS-10-B5)
广东省重点领域研发计划项目(2020B020219001)
作物种质资源保护与利用专项(NWB014)。
关键词
甘薯
爪哇镰刀菌
尖孢镰刀菌
毒力测定
sweet potato
Fusarium solani-melongenae
Fusarium oxysporum
toxicity test
