摘要
近年来,宫颈癌(cervical cancer)的中药治疗方法因其低毒高效的特点备受广大研究者的关注。Prx V(Peroxiredoxin V)是Prxs(Peroxiredoxins)蛋白家族中的重要成员,在调控氧化应激诱导的癌细胞增殖、迁移、集落形成、凋亡等过程中发挥着重要的作用。该研究集中探究了Prx V在苦木提取物(Picrasma quassioides extract,PQE)诱导人宫颈癌细胞(SiHa细胞)凋亡过程中的调控作用,通过构建Mock和Prx V过表达的SiHa细胞系,利用PQE进行处理,采用MTT assay实验、划痕实验、集落形成实验、荧光显微照相技术和蛋白质免疫印迹法对其进行细胞活力、增殖能力、细胞迁移能力、群落形成能力、线粒体损伤、细胞凋亡有关蛋白质的表达及细胞内活性氧(reactive oxygen species,ROS)水平变化等检测。研究结果显示,PQE通过下调细胞内Prx V蛋白的表达,从而有效促进细胞内ROS的蓄积,诱导SiHa细胞凋亡。另外,过量表达Prx V有效抑制了PQE引起的细胞内ROS的蓄积,进而有效降低了PQE介导的SiHa细胞凋亡及凋亡相关蛋白的表达水平。该研究初步揭示了Prx V在PQE诱导的宫颈癌SiHa细胞凋亡过程中的保护作用,为宫颈癌的临床中药治疗策略和Prx V的新功能研究提供了理论依据。
In recent years,traditional Chinese medicine treatments for cervical cancer have received more attentions due to their low toxicity and high efficiency.Prx V(Peroxiredoxin V),a major member of the Prxs(Peroxiredoxins)protein family,plays an important role in regulating the oxidative stress-induced cancer cell proliferation,migration,colony formation and apoptosis.The present study focused on the regulatory role of Prx V on PQE(Picrasma quassioides extract)induced apoptosis of human cervical cancer cells(SiHa cells),by constructing Mock and Prx V overexpressed SiHa cell lines.The cell viability,proliferation,migration,community formation,mitochondrial damage,apoptosis,intracellular ROS(reactive oxygen species)and apoptosis related protein expressions were investigated by MTT assay,scrape assay,colony formation assay,fluorescence micrography and Western blot.The results showed that PQE effectively increased intracellular ROS accumulation and induced apoptosis of SiHa cells by down-regulating the Prx V protein expression.In addition,overexpression of Prx V significantly reduced the PQE mediated intracellular ROS accumulations and apoptosis as well as the apoptosis-related proteins expression.These findings revealed that Prx V plays a protective role in PQE-induced apoptosis of cervical cancer SiHa cells,which provides a theoretical basis for exploring the clinical treatment strategy and the function of Prx V in cervical cancer.
作者
肖婉秋
张惠娜
任晨曦
郭晓宇
李楠
丛杰
冯耀元
孙虎男
XIAO Wanqiu;ZHANG Huina;REN Chenxi;GUO Xiaoyu;LI Nan;CONG Jie;FENG Yaoyuan;SUN Hunan(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2023年第1期35-43,共9页
Chinese Journal of Cell Biology
基金
2022年大学生创新创业训练计划(批准号:202210223020)资助的课题。
