摘要
为探索根系分隔方式对‘热研5号’圭亚那柱花草(Stylosanthes guianensis‘Reyan No.5’)亲缘识别与选择的影响,通过盆栽试验,研究圭亚那柱花草‘热研5号’分别与自身以及‘热研2号’、‘热研21号’和‘热研24号’3个品种伴生生长时,3种根系分隔方式(根系不分隔、尼龙网分隔、塑料膜分隔)下柱花草形态特征(株高、分枝数、根长、侧根数)和生物量分配(地上生物量、地下生物量、总生物量、叶茎比、根冠比)的变化差异。结果表明:不同根系分隔方式下,‘热研5号’圭亚那柱花草的生长指标发生显著变化,地上生物量、地下生物量、总生物量等表型指标均呈现出根系不分隔<尼龙网分隔<塑料膜分隔的变化趋势。根系不隔离条件下,与‘热研24号’伴生时,‘热研5号’圭亚那柱花草的株高、分枝数、地上生物量、地下生物量、总生物量、叶茎比均显著增加(P<0.05),与‘热研21号’伴生时,分枝数、侧根数、地下生物量、总生物量和根冠比显著增加(P<0.05),与‘热研2号’伴生时地上生物量、总生物量及叶茎比显著降低(P<0.05),其余生长指标与对照组相比均无显著差异;相互作用强度分析表明,‘热研5号’与‘热研24号’和‘热研21号’伴生时生长被促进,‘热研24号’的促进效应更强,与‘热研2号’伴生时生长被抑制。说明‘热研5号’圭亚那柱花草可以通过根系分泌物识别邻近植物与自身的亲缘关系,进而对邻近生长的近亲缘植株采取控制自身生长的合作性响应策略,对邻近生长的远缘亲属植株采取增强自身生长的竞争性响应策略。
This study assessed the effects of different root separation methods on kin recognition and selection of Stylosanthes guianensis‘Reyan No.5’.Three root separation methods were used:(i)no separation,(ii)nylon mesh,and(iii)plastic film.‘Reyan No.5’was grown alone and separately with three cultivars‘Reyan No.2’,‘Reyan No.21’,and‘Reyan No.24’,in pot experiments.Changes in the morphological characteristics(plant height,number of branches,root length,and number of lateral roots)and biomass allocation(aboveground biomass,underground biomass,total biomass,leaf-stem ratio,and root-shoot ratio)were recorded.The results showed that the growth indices of‘Reyan No.5’changed significantly under different root system separation methods.The aboveground,underground,and total biomass phenotypic indices showed a significant(P<0.05)trend of no separation between roots<nylon mesh separation between roots and plastic film separation between roots.With no root separation,the plant height,the number of branches,aboveground,underground,and total biomass indices,and the leaf-stem ratio of‘Reyan No.5’increased significantly(P<0.05)when it was grown with‘Reyan No.24’.The number of branches and lateral roots,underground and total biomass,and leaf-stem ratio improved significantly(P<0.05)when it was associated with‘Reyan No.21’.The aboveground and total biomass,and leaf-to-stem ratio of‘Reyan No.5’was significantly(P<0.05)reduced when it was grown with‘Reyan No.2’.Compared with the control group,the other growth indicators were not significantly different.The relative interaction index showed that the growth of‘Reyan No.5’was promoted when it grew with‘Reyan No.21’and‘Reyan No.24’,with‘Reyan No.24’having a stronger effect.The growth of‘Reyan No.5’was inhibited when grown with‘Reyan No.2’.These results suggest that‘Reyan No.5’could identify the relationship between neighboring plants and itself through root exudates and then adopts a cooperative response strategy of contro
作者
赵元辰
李雪枫
王俐媛
许超
ZHAO Yuanchen;LI Xuefeng;WANG Liyuan;XU Chao(Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants,Ministry of Education/College of forestry,Hainan University,Haikou 570228,Hainan,China)
出处
《草业科学》
CAS
CSCD
北大核心
2023年第2期491-501,共11页
Pratacultural Science
基金
国家自然科学基金(3226034、31860682)
海南省自然科学基金(319MS016、321RC471)。
关键词
亲缘选择
根系分隔
根系分泌物
相互作用强度
形态特征
生物量分配
竞争策略
kin selection
root separation
root exudates
relative interaction index
morphological characteristics
biomass allocation
competitive strategy
