摘要
目的分析自发性脑出血(spontaneous intracerebral hemorrhage,sICH)患者血清微小核糖核酸(microRNA,miR)-143,高迁移率族蛋白A2(high mobility group protein A2,HMGA2)的水平及其与预后的关系。方法选取2020年8月~2022年6月首都医科大学附属北京友谊医院平谷医院收治的129例sICH患者作为sICH组,根据sICH患者脑出血体积分为小血肿组50例(n=50,<10 ml)、中血肿组(n=45,10~30 ml)和大血肿组(n=34,>30 ml);根据sICH患者住院30天内是否死亡,将患者分为死亡组(n=40,预后不良)和存活组(n=89,预后良好),另选取同期来医院体检的131例健康者作为对照组。收集受试者临床资料,分别采用荧光定量PCR法和酶联免疫吸附法检测sICH患者入院24 h内、健康者体检时血清miR-143和HMGA2水平;分析sICH患者血清miR-143和HMGA2水平与美国国立卫生研究院卒中量表(NIHSS)、格拉斯哥昏迷量表(GCS)、Rankin量表(mRS)评分间的相关性,miR-143和HMGA2对sICH患者预后不良的预测价值以及影响sICH患者预后不良的危险因素。结果与对照组比较,sICH组血清miR-143水平(0.35±0.10 vs 1.03±0.07)降低,HMGA2水平(266.97±79.38mg/ml vs 153.13±40.24mg/ml)升高,差异具有统计学意义(t=63.599,14.618,均P<0.001)。小血肿组、中血肿组和大血肿组sICH患者血清miR-143水平(0.38±0.11,0.34±0.10,0.27±0.09)依次降低,HMGA2水平(212.01±78.72mg/ml,251.62±79.31mg/ml,368.11±80.44mg/ml)依次升高,差异具有统计学意义(F=11.895,40.424,均P<0.001)。与存活组比较,死亡组患者血清miR-143水平(0.30±0.06 vs 0.37±0.12)降低,HMGA2水平(316.81±80.03mg/ml vs 244.57±79.09mg/ml)升高,差异具有统计学意义(t=3.493,4.781,均P<0.05)。sICH患者血清miR-143水平与NIHSS,mRS评分呈负相关(r=-0.423,-0.498,均P<0.001),与GCS评分呈正相关(r=0.546,P<0.001)。HMGA2水平与NIHSS,mRS评分呈正相关(r=0.875,0.863,均P<0.001),与GCS评分呈负相关(r=-0.659,P<0.001)。血清miR-143,HMGA2及二者联合预测sICH患者�
Objective To analyze the levels of serum micro RNA(miR)-143 and high mobility group protein A2(HMGA2)in patients with spontaneous intracerebral hemorrhage(sICH)and their relationship with prognosis.Methods A total of 129 sICH patients admitted to Pinggu Hospital of Beijing Friendship Hospital Affiliated to Capital Medical University during August 2020 to June 2022 were included as the sICH group.According to the volume of intracerebral hemorrhage of sICH patients,they were divided into 50 of small hematoma group(n=50,<10 ml),medium hematoma group(n=45,10~30 ml),and large hematoma group(n=34,>30 ml).According to whether sICH patients died within 30 days of hospitalization,the patients were divided into death group(n=40,poor prognosis)and survival group(n=89,good prognosis).In addition,131 healthy people who came to the hospital for physical examination during the same period were selected as the control group.Clinical data of the subjects were collected.Fluorescence quantitative PCR and enzyme-linked immunosorbent assay were used to detect the serum levels of miR-143 and HMGA2 in sICH patients within 24 hours after admission and healthy subjects during physical examination.Analyze the correlation between serum miR-143 and HMGA2 levels and NIHSS,GCS and mRS scores in sICH patients,the predictive value of miR-143 and HMGA2 in poor prognosis of sICH patients and the risk factors affecting poor prognosis of sICH patients.Results Compared with the control group,the serum miR-143 level(0.35±0.10 vs 1.03±0.07)in sICH group was decreased,and the serum HMGA2 level(266.97±79.38mg/ml vs 153.13±40.24mg/ml)was increased,the differences were statistically significant(t=63.599,14.618,all P<0.001).The serum miR-143 level(0.38±0.11,0.34±0.10,0.27±0.09)of sICH patients in small hematoma group,medium hematoma group and large hematoma group was successively decreased,and the serum HMGA2 level(212.01±78.72mg/ml,251.62±79.31mg/ml,368.11±80.44mg/ml)was successively increased,and the differences were statistically significant(F
作者
李长宝
刘磊
宋保新
沈俊岩
张云鹏
LI Chang-bao;LIU Lei;SONG Bao-xin;SHEN Jun-yan;ZHANG Yun-peng(Department of Neurosurgery,Pinggu Hospital of Beijing Friendship Hospital Affiliated to Capital Medical University,Beijing 101200,China;Department of Neurosurgery,the First Medical Center of PLA General Hospital,Beijing 100853,China)
出处
《现代检验医学杂志》
CAS
2023年第2期69-74,共6页
Journal of Modern Laboratory Medicine
基金
北京市科学技术委员会科研计划项目(D191100000412004)。
