摘要
目的:观察微小RNA-128-3p(miR-128-3p)和高尔基体膜蛋白1(GOLM1)在肺腺癌细胞系中的表达特征,探讨miR-128-3p是否通过介导GOLM1调控肺腺癌细胞的增殖。方法:选择人肺癌细胞系SK-LU-1和A549、人永生化肺上皮细胞系16HBE,对SK-LU-1和A549分别建立miR-128-3p inhibitor组、siRNA-GOLM1组,并分别设立空白对照组,应用Western blot法检测GOLM1和增殖细胞核抗原(PCNA)的表达,应用实时荧光定量PCR法检测miR-128-3p的表达,应用CCK-8检测细胞增殖活性,挽救实验观察小干扰RNA-GOLM1逆转miR-128-3p inhibitor对PCNA表达的影响。结果:肺腺癌细胞系SK-LU-1和A549中miR-128-3p的表达量均明显低于16HBE,GOLM1和PCNA的表达量均明显高于16HBE(均P<0.05)。肺腺癌细胞系SK-LU-1和A549中,miR-128-3p inhibitior组中GOLM1的表达均明显高于空白对照组(均P<0.05),siRNA-GOLM1组的PCNA的表达量均明显低于空白对照组(均P<0.05),siRNA-GOLM1组在72 h开始细胞增殖活性均低于空白对照组(均P<0.05),均持续至96 h(均P<0.05),挽救实验显示干扰GOLM1可部分逆转miR-128-3p inhibitor对细胞增殖的促进作用(均P<0.05)。结论:肺腺癌细胞系中miR-128-3p低表达、GOLM1高表达,miR-128-3p靶向负调控GOLM1抑制肺腺癌细胞的增殖。
Objective:To observe the expression characteristics of microRNA-128-3p(miR-128-3p)and Golgi membrane protein 1(GOLM1)in lung adenocarcinoma cell lines,and to investigate whether miR-128-3p regulates lung adenocarcinoma cell proliferation by mediating GOLM1.Methods:Human lung cancer cell lines SK-LU-1 and A549 and human immortalized lung epithelial cell line 16HBE were selected.MiR-128-3p inhibitor group and siRNA-GOLM1 group were established respectively for SK-LU-1 and A549,and a blank control group was established respectively.GOLM1 and proliferating cell nuclear antigen(PCNA)were detected by Western blot,and miR-128-3p was detected by qRT-PCR,and the cell proliferation activity was detected by CCK-8.Results:The expression of miR-128-3p were significantly lower in both lung adenocarcinoma cell lines SK-LU-1 and A549 than that of 16HBE(all P<0.05),and the expressions of GOLM1 and PCNA were significantly higher in both lung adenocarcinoma cell lines SK-LU-1 and A549 than that of 16HBE(all P<0.05).GOLM1 was significantly higher in miR-128-3p inhibitior group than that of blank control group of SK-LU-1 and A549(all P<0.05).The expression of PCNA was significantly lower in the siRNA-GOLM1 group than that in the blank control group of SK-LU-1 and A549(all P<0.05).The cell proliferation activity was lower in the siRNA-GOLM1 group than that in the blank control group at 72 hours of SK-LU-1 and A549,and lasted until 96 hours(all P<0.05).The rescue experiments showed that interfering with GOLM1 could partially reverse the promotion effect of miR-128-3p inhibitor on cell proliferation(all P<0.05).Conclusion:MiR-128-3p is lowly expressed and GOLM1 is highly expressed in lung adenocarcinoma cell lines.MiR-128-3p inhibits the proliferation of lung adenocarcinoma cells by targeting GOLM1.
作者
崔文馨
赵晓辉
高蔚然
王娜
CUI Wenxin;ZHAO Xiaohui;GAO Weiran;WANG Na(Outpatient Blood Collection Room,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China)
出处
《陕西医学杂志》
CAS
2023年第4期385-389,共5页
Shaanxi Medical Journal
基金
中国医疗手牵手工程委员会-北京医学奖励基金会资助项目(YXJL-2021-1092-0640)。
