摘要
背景:静电纺丝和富血小板血浆均在组织再生与修复方面展现出巨大的应用前景,而对于负载富血小板血浆的静电纺丝纳米纤维复合支架的研究并不多见。目的:综述近年来负载富血小板血浆的静电纺丝纳米纤维复合支架在骨及软组织工程中的研究进展,以期为复合支架后续的深入研究和临床应用提供思路与理论支持。方法:以“Tissue engineering,Platelet-rich plasma,Electrospun scaffolds,Stem cell,Regenerative medicine”为英文检索词,以“组织工程、富血小板血浆、静电纺丝、干细胞、组织再生、骨再生、软骨再生、软组织再生”作为中文检索词,在PubMed,Web of Science,Ovid,SpringerLink,Wiley Oline Library,Medline,万方和中国知网数据库进行检索,最终纳入64篇文献进行综述。结果与结论:(1)负载富血小板血浆的静电纺丝纳米复合支架兼顾富血小板血浆与静电纺丝纳米支架的优点,更具弹性,不易撕裂,具备适当力学强度,生物相容性良好。(2)将富血小板血浆与三维取向纤维支架结合可更好地模拟天然组织的微环境,能更好地促进细胞的生长、增殖和分化,促进骨及软组织的修复与再生。(3)在骨组织再生方面,能在不同应力条件下有一定的抗压能力,具有良好细胞相容性;(4)在软组织再生方面,通过调控材料的孔隙率和表面积,既有益于细胞呼吸,又可以促进伤口愈合、神经细胞的生长和血管再生。(5)然而,目前能应用于临床的理想的能促进骨及软组织再生修复的材料仍然较少,如何利用各种材料的优点及如何优化各种材料的理化性能,从而加强支架与种子细胞的相互作用,使其对富血小板血浆源性生长因子保持较高的缓释效能,仍然是未来研究亟待解决的难题之一,有待进一步的体内研究和临床试验。
BACKGROUND:Both electrospinning and platelet-rich plasma have shown great application prospects in tissue regeneration and repair,but there are few studies on electrospun nanofiber composite scaffolds loaded with platelet-rich plasma.OBJECTIVE:To review the research progress of electrospun nanofiber composite scaffolds loaded with platelet-rich plasma in bone and soft tissue engineering in recent years,in order to provide ideas and theoretical support for further in-depth research on the clinical application of composite scaffolds.METHODS:Using“tissue engineering,platelet-rich plasma,electrospun scaffolds,stem cell,regenerative medicine”as English search terms and“tissue engineering,platelet-rich plasma,electrospinning,stem cell,tissue regeneration,bone regeneration,cartilage regeneration,soft tissue regeneration”as Chinese search terms,we searched PubMed,Web of Science,Ovid,SpringerLink,Wiley Oline Library,Medline,Wanfang,and CNKI databases.Totally 64 articles were finally included for review.RESULTS AND CONCLUSION:(1)Electrospun nanoscaffolds loaded with platelet-rich plasma have the advantages of both platelet-rich plasma and electrospun nanoscaffolds,which are more elastic,less tearing,suitable mechanical strength and good biocompatibility.(2)The combination of platelet-rich plasma and three-dimensional oriented fiber scaffolds can better simulate the microenvironment of natural tissues,promote cell growth,proliferation and differentiation,and promote bone and soft tissue repair and regeneration.(3)In terms of bone tissue regeneration,it has certain compressive capacity under different stress conditions,and has good cell compatibility.(4)In terms of soft tissue regeneration,by regulating the porosity and surface area of the material,it is beneficial to cell respiration,and can promote wound healing,nerve cell growth and vascular regeneration.(5)However,due to various reasons,there are still few ideal materials that can be used in clinical practice to promote the regeneration and repair of bone and sof
作者
赵明月
杨顺
涂希玲
高丽
杨琨
刘琪
Zhao Mingyue;Yang Shun;Tu Xiling;Gao Li;Yang Kun;Liu Qi(Zunyi Medical University,Zunyi 563000,Guizhou Province,China;Department of Periodontics,Affiliated Stomatological Hospital of Zunyi Medical University,Zunyi 563000,Guizhou Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第34期5554-5560,共7页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金项目(82060204),项目负责人:高丽
遵义市市级重点项目(市15851),项目负责人:刘琪
贵州省卫健委科学技术基金项目(gzwkj2022-169),项目负责人:杨琨
贵州省科学技术基金计划(黔科合基础[2020]1Y328),项目负责人:杨琨。
关键词
富血小板血浆
静电纺丝
干细胞
组织再生
骨再生
软骨再生
软组织再生
综述
组织工程
platelet-rich plasma
electrospinning
stem cell
tissue regeneration
bone regeneration
cartilage regeneration
soft tissue regeneration
review
tissue engineering
