摘要
背景:当前使用的传统试剂(甲醛溶液固定、乙醇脱水、二甲苯透明脱蜡)对组织前期处理制作的常规石蜡切片质量较低,已成为制约脂肪细胞性肿瘤病理诊断的瓶颈。而且,传统试剂容易对实验室环境造成污染,对实验室人员造成多种健康危害。目的:探讨环保型组织样本制备液在脂肪细胞肿瘤苏木精-伊红染色及非典型性脂肪瘤性肿瘤/高分化脂肪肉瘤MDM2基因检测中的应用价值。方法:选取2016年2月至2022年7月期间592例脂肪细胞肿瘤标本为研究对象,同一标本对半切开,按照所使用的标本前期处理试剂的不同,随机分为2组:传统组和环保组。传统组使用传统试剂甲醛溶液固定、乙醇脱水、二甲苯透明脱蜡制作常规石蜡切片592张;环保组使用环保型组织样本制备液制作切片592张。依据切片苏木精-伊红染色质量的不同等级,比较两组切片的苏木精-伊红染色优良率;进一步对其中病理确诊为非典型性脂肪瘤性肿瘤/高分化脂肪肉瘤的33例标本再次切片后,使用荧光原位杂交法检测MDM2基因,比较两组切片MDM2基因扩增率的差异。结果与结论:(1)相对于传统组,环保组的脂肪细胞肿瘤的组织切片更舒展、更完整,细胞无折叠,染色更清晰,红蓝对比度更佳,细胞结构更致密;(2)环保组的组织切片苏木精-伊红染色优良率高于传统组,差异有显著性意义(P=0.000);(3)相对于传统组,环保组的MDM2探针和CSP12探针仅在标本染色体特定的区域内结合,更具特异性;(4)环保组杂交成功细胞数高于传统组,差异有显著性意义(P=0.000);(5)传统组和环保组的MDM2基因信号数、MDM2/细胞值、CSP12信号数、CSP12/细胞值、MDM2/CSP12值之间的差异无显著性意义(P>0.05);(6)两组MDM2基因扩增率比较,差异无显著性意义(P=0.31);(7)结果表明,环保型组织样本制备液有利于提高脂肪细胞肿瘤的苏木精-伊红染色优良率,保证了非典型�
BACKGROUND:The quality of conventional paraffin sections prepared by traditional reagents(formaldehyde solution fixation,ethanol dehydration,xylene transparent dewaxing)used for tissue pretreatment is low,which has become the bottleneck of pathological diagnosis of adipocytic tumors.Moreover,traditional reagents are easy to pollute the laboratory environment and cause various health hazards to laboratory personnel.OBJECTIVE:To explore the application value of environmental friendly tissue sample preparation solution in the detection of MDM2 gene in adipocyte-tumor hematoxylin-eosin staining and atypical lipomatous-tumor/well differentiated liposarcoma.METHODS:From February 2016 to July 2022,592 samples of adipocyte tumors were selected as the research objects.The same sample was cut in half.According to the different reagents used in the pretreatment of samples,they were randomly divided into two groups:the traditional group and the environmental protection group.In the traditional group,592 conventional paraffin sections were made by formalin fixation,alcohol dehydration and xylene transparent dewaxing.In the environmental protection group,592 slices were made with environmental protection tissue sample preparation solution.According to the different grades of hematoxylin-eosin staining quality of sections,the excellent and good rate of the matoxylin-eosin staining was compared between the two groups.Further,33 specimens of atypical lipomatous tumor/well differentiated liposarcoma confirmed by pathology were sectioned again,and the MDM2 gene was detected by fluorescence in situ hybridization to compare the difference in the amplification rate of MDM2 gene between the two groups.RESULTS AND CONCLUSION:(1)Compared with the traditional group,the tissue sections of the adipocyte tumors in the environmental protection group were more stretched and complete;the cells were not folded;the staining was clearer;the red blue contrast was better and the cell structure was denser.(2)The excellent and good rate of hematoxylin
作者
陈洪才
王少洪
王媛媛
古家美
刘春鹏
赵勇强
邱晓阳
詹晓芬
Chen Hongcai;Wang Shaohong;Wang Yuanyuan;Gu Jiamei;Liu Chunpeng;Zhao Yongqiang;Qiu Xiaoyang;Zhan Xiaofen(Department of Pathology,Shantou Central Hospital,Shantou 515031,Guangdong Province,China;Department of Molecular Diagnosis,Cancer Center,Sun Yat-sen University,Guangzhou 510060,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第34期5510-5515,共6页
Chinese Journal of Tissue Engineering Research
基金
汕头市科技计划医疗卫生类别项目(汕府科[2017]119号-18),项目负责人:詹晓芬
汕头市科技计划医疗卫生类别项目(汕府科[2020]5号-41),项目负责人:邱晓阳。
