摘要
目的 检测血清外泌体miR-15b、miR-16、miR-21和miR-31在结直肠癌患者中的表达水平,并评估其对结直肠癌的诊断价值。方法 选取2018年3月至2022年5月本院收治的123例直肠癌患者(结直肠癌组)及117例大肠腺瘤患者作为研究对象(结直肠癌组),并纳入150例健康对照者作为对照(健康对照组)。提取血清外泌体中miR-15b、miR-16、miR-21和miR-31,qRT-PCR比较4种miRNA在各组中表达水平差异。受试者工作特征(ROC)曲线评价4种miRNA单一及组合panel对结直肠癌的诊断价值。结果 结直肠癌组血清miR-15b、miR-16、miR-21和miR-31水平高于健康对照组(P <0.05),且miR-15b、miR-21和miR-31水平高于结直肠腺瘤组(P <0.05)。Ⅲ-Ⅳ期、伴淋巴结浸润、低分化程度结直肠癌组患者血清miR-15b、miR-21和miR-31水平分别高于Ⅰ-Ⅱ期、不伴淋巴结浸润、中-高分化程度患者,miR-15b、miR-21和miR-31水平与临床TNM分期、伴淋巴结浸润呈正相关,与分化程度呈负相关。4种miRNA中诊断价值最高的指标是miR-15b,其敏感度和特异度分别为81.33%和91.80%(P <0.05);且miR-15b、miR-21和miR-31的组合panel具有更好的诊断价值,其敏感度和特异度分别为95.06%和94.44%(P <0.05)。结论 miR-15b、miR-16、miR-21和miR-31在结直肠癌患者外周血血清中异常表达,miR-15b、miR-21和miR-31水平与结直肠癌的发病及进展密切相关,且miR-15b、miR-21和miR-31组合panel具有作为结直肠癌的新型诊断模型的潜力。
Objective To evaluate the expression levels of serum exosomes miR-15b,miR-16,miR-21 and miR-31 in patients with colorectal cancer,and the diagnostic value of single or combined panel for colorectal cancer.Methods A total of 123 patients with colorectal cancer and 117 patients with colorectal adenoma admitted to our hospital from March 2018 to May 2022 were selected as the research objects,and 150 healthy controls were included as the control group.Serum exosomes were extracted and identified,and miR-15b,miR-16,miR-21 and miR-31 in serum exosomes were further extracted and measured by qRT-PCR.The expression levels of the four miRNAs in each group were compared.The receiver operating characteristic(ROC)curve was drawn to evaluate the diagnostic value of single and combined panel of four miRNAs for colorectal cancer.Results The serum levels of miR-15b,miR-16,miR-21 and miR-31 in colorectal cancer group were higher than those in healthy control group,and the levels of miR-15b,miR-21 and miR-31 were higher than those in colorectal adenoma group(P<0.05).The serum levels of miR-15b,miR-21 and miR-31 in stageⅢ-Ⅳpatients with lymph node invasion and low differentiation were higher than those in stageⅠ-Ⅱpatients without lymph node invasion and moderate to high differentiation,respectively.The levels of miR-15b,miR-21 and miR-31 were positively correlated with clinical TNM stage and lymph node infiltration,and negatively correlated with the degree of differentiation.Among the four miRNAs,miR-15b was the best index to distinguish rectal cancer from normal subjects,and the sensitivity and specificity were 81.33%and 91.80%,respectively(P<0.05).Besides,the combined panel of miR-15b,miR-21 and miR-31 can better distinguish between healthy control group and colorectal cancer.The sensitivity and specificity were 95.06% and 94.44%,respectively(P < 0.05). Conclusion miR-15b,miR-16,miR-21 and miR-31 wereabnormally expressed in the peripheral blood serum of patients with colorectal cancer,and the levels of miR-15b,miR-21 and m
作者
郝剑
韩磊
HAO Jian;HAN Lei(Department of Clinical Laboratory,the First Affiliated Hospital of Hebei North Medical College,Zhangjiakou 050051,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2023年第3期369-373,共5页
The Journal of Practical Medicine
基金
河北省医学科学研究重点课题计划项目(编号:20180876)。
