摘要
为揭示冬油菜耐盐性的差异,以白菜型冬油菜陇油16号、天油2号、天油4号和甘蓝型冬油菜宁油2号为材料,采用常规纸上萌发试验,用NaCl溶液(34、68、102、136、170 mmol·L^(-1))模拟盐胁迫,研究NaCl胁迫对冬油菜种子萌发和相关生理指标的影响,用相关性分析评价耐盐性指标,用回归分析确定耐盐临界值。结果表明:与对照相比,随着NaCl浓度的升高,冬油菜种子发芽率、胚根长、胚芽长和幼苗干重、鲜重均呈下降趋势,且对白菜型冬油菜的影响程度小于甘蓝型冬油菜,高浓度NaCl(170 mmol·L^(-1))显著降低冬油菜胚根长;幼苗脯氨酸含量、细胞膜透性、丙二醛(MDA)含量和过氧化物酶(POD)活性呈逐渐升高趋势。相关分析显示:冬油菜种子发芽率与胚根长、胚芽长、幼苗鲜重和干重呈极显著(P<0.01)正相关,与MDA含量、细胞膜透性、脯氨酸含量和POD活性呈极显著(P<0.01)负相关;冬油菜幼苗MDA含量与细胞膜透性、脯氨酸含量呈极显著(P<0.01)正相关。回归分析表明:冬油菜种子萌发生长指标的耐盐临界值为164.87~251.99 mmol·L^(-1),半致死浓度和致死浓度分别是316.29~494.95 mmol·L^(-1)和558.57~883.67 mmol·L^(-1),冬油菜萌发生长指标的耐盐临界值由高到低是发芽率、胚芽长、幼苗鲜重、幼苗干重、胚根长。冬油菜品种间耐盐性强弱为陇油16号>天油2号>天油4号>宁油2号,冬油菜品种间盐害指数大小为宁油2号>天油4号>天油2号>陇油16号。综上可知,可用种子发芽率、胚根长和丙二醛含量作为冬油菜品种耐盐性筛选指标,研究结果可为耐盐冬油菜种质创新提供参考。
In this study,we investigated the salt tolerance of winter oilseed rape cultivars by subjecting Brassica rapa cultivars of Longyou 16,Tianyou 2,Tianyou 4,and Brassica napus cultivars of Ningyou 2 to NaCl stress at concentration of 34,68,102,136,170 mmol·L^(-1).We measured the seed germination indexes and physiological characteristics.In addition,salt tolerance index and salt tolerance critical value were determined by correlation analysis and regression analysis,respectively.The results showed that seed germination rate,radicle length,sprout length,seedling dry weight and fresh weight all showed a decreasing trend with the increase of NaCl concentration,and the decrease of Brassica rapa cultivars of Longyou 16 and Tianyou 2 and Tianyou 4 was less than that of Brassica napus cultivars of Ningyou 2,and the inhibition degree of high salt concentration(170 mmol·L^(-1))on radicle was significant.With increasing salt stress concentration,proline content,cell membrane permeability,malondialdehyde(MDA)content and peroxidase(POD)activity of seedlings gradually increased.Correlation analysis showed that seed germination rate was significantly(P<0.01)positively correlated with radicle length,sprout length,seedling fresh weight and seedling dry weight,while germination rate was significantly(P<0.01)negatively correlated with MDA content,cell membrane permeability,proline content and POD activity;MDA content in rapeseed seedlings was significantly(P<0.01)positively correlated with cell membrane permeability and proline content.Regression analysis showed that critical value of salt tolerance for seed germination and growth index of winter rapeseed was 164.87-251.99 mmol·L^(-1),and the median lethal concentration and lethal concentration were 316.29-494.95 mmol·L^(-1) and 558.57-883.67 mmol·L^(-1),respectively.The critical value of germination growth index of winter rape showed germination rate>sprout length>fresh weight of seedlings>dry weight of seedlings>radicle length.The salt tolerance of winter oilseed rape showed Lo
作者
许耀照
曾秀存
王振朝
党仕卓
刘永晶
XU Yaozhao;ZENG Xiucun;WANG Zhenchao;DANG Shizhuo;LIU Yongjing(College of Agriculture and Ecological Engineering,Hexi University,Zhangye 734000,Gansu,China;Key Laboratory of Hexi Corridor Resources Utilization of Gansu,Zhangye 734000,Gansu,China;School of Life Science and Engineering,Hexi University,Zhangye 734000,Gansu,China)
出处
《浙江农业学报》
CSCD
北大核心
2023年第3期499-508,共10页
Acta Agriculturae Zhejiangensis
基金
甘肃省教育厅青年博士基金(2021QB-113)
国家自然科学基金(31560397,31660401)
河西学院博士启动基金(KYQD2022009)。
关键词
NACL胁迫
冬油菜
种子萌发
生理特性
耐盐临界值
耐盐指标
NaCl stress
winter rapeseed
seed germination
physiological characteristic
salt tolerance critical value
salt tolerance index
