摘要
抗生素抗性基因作为一种新型“污染物”已经受到广泛关注.研究边缘海中抗生素抗性基因和人类致病菌的组成特征,有助于深入认识海洋环境中抗生素抗性基因的起源和海洋致病菌的潜在健康风险.使用基于高通量测序的宏基因组学分析方法在中国边缘海沉积物中发现了多种抗生素抗性基因,主要以多重耐药基因为主,其中南海沉积物中抗生素抗性基因的总丰度约是黄渤海的2倍.与中国边缘海相比,珠江口沉积物中抗生素抗性基因丰度更高,与人类常用抗生素(如磺胺类、氨基糖苷类、β-内酰胺类、四环素类等)的耐药性更为相关.此外,中国边缘海沉积物中几乎没有发现质粒携带的抗性基因,而珠江口有约20%的抗性基因由质粒携带.中国边缘海沉积物中主要人类致病菌种包括肺炎链球菌(24.4%)、肺炎克雷伯菌(19.9%)、无乳链球菌(9.2%)、铜绿假单胞菌(6.9%)等.珠江口沉积物中致病菌群落组成与中国边缘海显著不同,副溶血性弧菌和空肠弯曲杆菌的丰度相对更高.上述研究表明,中国边缘海沉积物中存在高多样性的抗生素抗性基因和人类致病菌,人类活动干扰可导致其污染水平提高.
Antibiotic resistance genes(ARGs)are highly concerned as a group of emerging“contaminants”.Characterization of ARGs and putative human pathogenic bacteria(HPB)in the marginal sea is essential for understanding the origin of ARGs and the potential health risks posed by oceanic pathogenic bacteria.Multiple ARGs were found in the sediments of Chinese marginal seas using high-throughput sequencing-based metagenomic approaches,with multidrug being the predominant resistance type.Herein,total ARG abundance in the sediments of South China Sea were approximately 2 times higher than those in the sediments collected from the Bohai Sea and Yellow Sea.Compared to Chinese marginal seas,ARGs in the sediments of the Pearl River Estuary(PRE)are more abundant and closely associated with anthropogenic antibiotics such as sulfonamides,aminoglycosides,β-lactams,tetracyclines,etc.ARGs possibly carried by plasmids were hardly detected in the sediments of Chinese marginal seas,but could account for approximately 20%of the total ARGs in the PRE sediments.The prevalent putative HPB in the sediments of Chinese marginal seas were Streptococcus pneumoniae(24.4%),Klebsiella pneumoniae(19.9%),Streptococcus agalactiae(9.2%),Pseudomonas aeruginosa(6.9%),etc.The community composition of putative HPB in the PRE sediments was substantially different from those in the Chinese marginal seas,which were featured with the relatively higher ratios of Vibrio parahaemolyticus and Campylobacter jejuni.Our results suggest that diverse ARGs and putative HPB occur in the sediments of Chinese marginal seas,and anthropogenic impacts may increase their pollution levels.
作者
李昭宏
谢秀琴
原珂
杨颖
黄永顺
陈保卫
林岚
LI Zhaohong;XIE Xiuqin;YUAN Ke;YANG Ying;HUANG Yongshun;CHEN Baowei;LIN Lan(Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering,School of Marine Science,Sun Yat-sen University,Zhuhai,519082,China;Zhujiang Hospital of Southern Medical University,Guangzhou,510282,China;Guangdong Provincial Hospital for Occupational Diseases Prevention and Treatment,Guangzhou,510300,China)
出处
《环境化学》
CAS
CSCD
北大核心
2023年第3期805-812,I0002,共9页
Environmental Chemistry
基金
国家重点研发计划“政府间国际科技创新合作”重点专项项目(2022YFE0103200)
国家自然科学基金(42276152)资助..
关键词
中国边缘海
抗生素抗性基因
致病菌
宏基因组学
沉积物
Chinese marginal seas
antibiotic resistance genes
pathogenic bacteria
metagenomics
sediments
