摘要
新型冠状病毒(severeacute respiratory syndrome coronavirus 2,SARS-CoV-2)核酸检测是迅速发现传染源、锁定管控目标,进而切断传播途径的关键有效手段。成簇规律间隔短回文重复序列(clustered regularly interspaced short palindromic repeat,CRISPR)及其相关蛋白(CRISPR associated protein,Cas)系统的定向基因编辑技术在病原微生物检测方面有广阔的应用前景,尤其是分别靶向切割RNA和DNA的Cas13和Cas12,依靠其附带切割特性,结合荧光报告系统,已应用于SARS-CoV-2的核酸检测,在灵敏度、专一性、便携性、经济性和易操作性等多方面表现出色。此文系统总结了基于CRISPR-Cas系统的SARS-CoV-2核酸检测技术研究进展,包括作用原理、改进的方案和优势,以评价推广应用的可行性,也为基于CRISPR-Cas系统研发病原体快速检测方法提供参考。
Nucleic acid testing of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a critical and effective measure to quickly identify the source of infection,control the infected cases and block the transmission route.The capability of clustered regularly interspaced short palindromic repeat(CRISPR)and CRISPR associated protein(Cas)system to edit targeted gene presents broad application prospects in pathogen detection,especially Cas13 and Cas12,proteins targeting RNA and DNA,respectively.Relying on their collateral cleavage activity with fluorescent reporter systems,they have been utilized to detect SARSCoV-2 nucleic acid and perform remarkably in sensitivity,specificity,portability,economy and ease of operation.This paper summarizes systematically the technology progress for SARS-CoV-2 detection based on CRISPR-Cas system,including mechanisms,improvement measures and their advantages,to evaluate the feasibility of the technology application.This also provides a reference for developing pathogen detection tools based on CRISPR-Cas system.
作者
刘彬
栾洁
巴剑波
Liu Bin;Luan Jie;Ba Jianbo(Laboratory of Human Factors of Navigation and Effect Prevention of High Technology Weapons Device,Naval Medical Center,Shanghai 200433,China)
出处
《国际生物制品学杂志》
CAS
2023年第1期27-32,共6页
International Journal of Biologicals
基金
国家重点研发计划(2022YFC2602900)
关键词
新型冠状病毒
成簇规律间隔短回文重复序列及其相关蛋白系统
核酸检测
Severe acute respiratory syndrome coronavirus 2
Clustered regularly interspaced short palindromic repeat and associated protein system
Nucleic acid testing
