摘要
本试验旨在比较青藏高原中华羊茅不同部位(全株、茎、叶和穗)的营养价值及分子结构特征,为建立高原牧草营养价值快速评定技术和家畜饲粮配方设计提供基础数据。试验采用概略养分分析法和康奈尔净碳水化合物-蛋白质体系(CNCPS)分析中华羊茅不同部位的营养价值,同时利用傅立叶变换红外光谱(FTIR)技术对不同部位的分子结构进行研究,并探求它们之间的相关关系。结果表明:1)中华羊茅不同部位蛋白质组分和蛋白质分子结构存在显著差异(P<0.05)。其中,穗的粗蛋白质(CP)、中性洗涤不溶粗蛋白质(NDICP)、难溶性真蛋白质(PB1)、酰胺Ⅱ区峰高、α-螺旋峰高、β-折叠峰高以及α-螺旋峰高/β-折叠峰高值均为最高,茎的上述指标较低;不同部位的蛋白质组分与蛋白质分子结构参数存在相关关系。2)中华羊茅不同部位的碳水化合物组分和碳水化合物分子结构存在显著差异(P<0.05)。其中,穗的淀粉和非纤维性碳水化合物(NFC)含量最高;茎的中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、酸性洗涤木质素(ADL)和不可消化纤维(CC)含量最高;茎的结构性碳水化合物峰面积、纤维素复合物峰面积和峰高显著高于全株和其他部位(P<0.05);中华羊茅不同部位的碳水化合物组分与其分子结构参数存在较强的相关关系。综上所述,中华羊茅各部位的营养价值和分子结构特征存在显著差异;以中华羊茅为主要粗饲料来源设计饲粮配方时,应注意搭配补充蛋白质饲料并充分考虑饲粮的可消化性;下一步可利用本研究获取的基础数据,探索建立中华羊茅营养价值的快速评定技术。
The objective of this study was to compare the nutritive value and molecular structure characteristics in different parts(i.e.whole plant,stem,leaf and ear)of Festuca sinensis from Qinghai-Tibet plateau and provide the basic data for the establishment of rapid evaluation technology of nutrient value of plateau forage and the design of livestock diet formula.Conventional nutrient analysis and Cornell net carbohydrate and protein system(CNCPS)were applied to analyze the nutritional value in different parts of Festuca sinensis,at the same time,Fourier transform infrared spectroscopy(FTIR)technology was used to study the molecular structure of different parts and explore the correlation between them.The results showed as follows:1)there were significant differences in protein components and molecular structure in different parts of Festuca sinensis(P<0.05).Among them,the contents of crude protein(CP),neutral detergent insoluble crude protein(NDICP),insoluble true protein(PB 1),amideⅡpeak height,α-helix peak height,β-helix peak height andα-helix peak height toβ-helix peak height ratio were highest in ear,while those in stem were lower.Correlations were found between the protein molecular structural parameters and protein components.2)There were significant differences in carbohydrate components and carbohydrate molecular structure in different parts of Festuca sinensis(P<0.05).Among them,the contents of starch and non-fiber carbohydrate(NFC)in ear were the highest,the contents of neutral detergent fiber(NDF),acid detergent fiber(ADF),acid detergent lignin(ADL)and indigestible fiber(CC)in stem were the highest,and the structural carbohydrate peak area,the peak area and peak height of cellulose complex in stem were significantly higher than those in the whole plant and the other parts(P<0.05).The carbohydrate components in different parts of Festuca sinensis had strong correlation with its molecular structure parameters.In conclusion,there are significant differences in the nutritional value and molecular structur
作者
孙晓旭
彭婧
江雨霏
史海涛
陈有军
蔡晓蕊
黄艳玲
李胜利
SUN Xiaoxu;PENG Jing;JIANG Yufei;SHI Haitao;CHEN Youjun;CAI Xiaorui;HUANG Yanling;LI Shengli(Key Laboratory of Qinghai-Tibet Plateau Animal Genetic Resource and Utilization,Ministry of Education,College of Animal&Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China;Collaborative Innovation Center for Ecological Animal Husbandry of Qinghai-Tibetan Plateau,Institute of Qinghai-Tibetan Plateau,Southwest Minzu University,Chengdu 610041,China;State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2023年第3期1850-1862,共13页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(31902187)
四川肉羊创新团队建设专项经费(CXTD2019-14)
农业农村部高海拔地区奶牛适应性生产技术试验示范项目
国家民委中青年英才项目(2020)
国家重点研发计划(2022YFD1601605)。
