摘要
植物DUR3同源蛋白属于钠离子/溶质共运蛋白家族的尿素高亲和力运输蛋白,在植物体对外源尿素的主动吸收及内源尿素的再分配过程中具有重要作用。为明确棉花DUR3基因的结构和进化情况,基于生物信息学的方法,从全基因组水平鉴定陆地棉和雷蒙德氏棉的DUR3基因,并对基因结构、跨膜结构域、基序分布、进化关系等进行分析。结果表明:(1)从陆地棉A亚组和D亚组染色体各鉴定出1个DUR3基因,从雷蒙德氏棉基因组鉴定出1个DUR3基因。这3个棉花DUR3同源蛋白同其他植物DUR3同源蛋白一样,具有15个跨膜结构域,具有3个位置一致、高度保守的基序。(2)基因结构分析表明,双子叶植物DUR3基因的外显子个数明显多于单子叶植物,这3个棉花DUR3基因的外显子个数亦是如此。(3)根据物种间种属亲缘关系,对不同物种DUR3氨基酸序列构建的进化树显示,棉花的同双子叶植物的聚在一起。(4)DUR3直系同源基因和旁系同源基因的K a/K s比值普遍均大于1,说明这些基因在进化过程中主要受到正向选择的作用。该研究结果为深入研究棉花DUR3同源蛋白提供了理论基础。
Plant DUR3 homologous protein is a high affinity urea transporter which belongs to the family of sodium/solute symporter family,and plays an important role in the active absorption of exogenous urea and redistribution of endogenous urea by plants.The purpose of this study was to clarify structure and evolution situation of cotton DUR3 gene.Based on bioinformatics methods,DUR3 genes were identified from Gossypium hirsutum and G.raimondii genomic sequences,and then the gene structure,transmembrane domain,motif location,as well as phylogenetic relationship,were systematically analyzed.The results were as follows:(1)Three DUR3 genes were identified from the A and D subgroup chromosomes of upland cotton and G.raimondii genomic sequences.These three cotton DUR3 homologous proteins,like other plant DUR3 homologous proteins,had 15 transmembrane domains and three highly conserved motifs with consistent positions.(2)The gene structure analysis showed that the number of exons of DUR3 genes in dicotyledons was significantly higher than that in monocotyledons,and so were the cotton DUR3 genes.(3)Phylogenetic analysis revealed that the amino acid sequences of different species were classified according to species kinship,and cotton clustered in one branch with dicotyledons.(4)The K a/Ks values of orthologous and paralogous DUR3 genes were generally more than one,indicating that those genes mainly experienced positive selection among evolution.The results of this study provide a theoretical basis for further research on cotton DUR3 homologous protein.
作者
巩元勇
赵丽华
闫飞
孙伊辰
王慧
刘来华
GONG Yuanyong;ZHAO Lihua;YAN Fei;SUN Yichen;WANG Hui;LIU Laihua(Biological and Chemical Engineering College,Panzhihua University,Panzhihua 617000,Sichuan,China;Key Laboratory of Plant-Soil Interactions,Ministry of Education,Center for Resources,Environment and Food Security,China Agricultural University,Beijing 100193,China)
出处
《广西植物》
CAS
CSCD
北大核心
2023年第2期283-292,共10页
Guihaia
基金
国家自然科学基金(31301682)
金沙江干热河谷生态修复与治理创新研究团队专项经费(035200179)。
