摘要
青海草原毛虫(Gynaephora qinghaiensis)是危害青藏高原高寒草甸最重要的害虫之一。本研究通过第二代高通量测序技术对青海草原毛虫4龄幼虫、雌雄成虫进行转录组测序并进行基因功能注释,同时筛选获得其气味结合蛋白基因(GqinOBPs)并进行生物信息学分析。结果表明:转录本经过组装之后总计得到63335条unigenes,在全部unigenes中筛选并鉴定出17个GqinOBPs基因,通过生物信息学分析发现这些气味结合蛋白为热稳定性蛋白,亚细胞定位主要在胞外,二三级结构主要由α螺旋和无规则卷曲组成,多序列比对显示GqinOBPs具有昆虫OBPs的半胱氨酸模式识别序列;系统分析表明,GqinOBPs基因与同是鳞翅目昆虫的桃蛀螟、小线角木蠹蛾和沙棘木蠹蛾亲缘关系较近。该研究首次公开并分析青海草原毛虫转录组数据,同时挖掘出其气味结合蛋白基因,为进一步研究青海草原毛虫的基因功能奠定基础。
The Gynaephora qinghaiensis is one of the most harmful pests endangering the alpine meadows on the Qinghai-Tibet Plateau.In this study,transcriptome sequencing of fourth-instar larvae,male and female adults of G.qinghaiensis was performed by second-generation high-throughput sequencing;and the odorant binding protein(GqinOBPs)genes were selected further underwent bioinformatics analysis.The results showed that 17 GqinOBPs genes were screened and identified among a total of 63335 assembled unigenes.These OBPs were revealed to be thermostable and displays an extracellular localization by bioinformatics analysis.The secondary and tertiary structure consists mainly ofαhelices and an irregular coil.Multiple sequence alignment supported that GqinOBPs showed conserved constitution pattern of cysteine residues as in most insects.Evolutionarily,GqinOBPs gene is closely relative to the Dichocrocis punctiferalis,Holcocerus insularis Staudinger and Holcocerus hippophaecolus,which are also lepidoptera counterparts.This study first reported the transcriptomic profiling of the G.qinghaiensis,and the identification of the odor-binding protein genes,which would lay out a foundation for further study on functions of the genes in the G.qinghaiensis.
作者
南彦斌
张月
何啟玥
陈帅
周渊涛
NAN Yan-bin;ZHANG Yue;HE Qi-yue;CHEN Shuai;ZHOU Yuan-tao(School of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai Province 810016,China)
出处
《草地学报》
CAS
CSCD
北大核心
2023年第3期688-698,共11页
Acta Agrestia Sinica
基金
青海省科技厅青年基金项目,草原毛虫嗅觉感受分子机理的研究(2022-ZJ-949Q)资助。
关键词
青海草原毛虫
转录组测序
气味结合蛋白
生物信息学分析
系统发育树
Gynaephora qinghaiensis
Transcriptome sequencing
Odorant binding protein
Bioinformatics analysis
Phylogenetic tree
