摘要
采用菌丝生长速率法测定了3种中药乙醇提取物对猕猴桃葡萄座腔菌(Botryosphaeria dothidea)的毒力以及联合作用。结果表明:苦参、丹参以及黄岑片3种乙醇提取物对B.dothidea菌丝生长均有一定的抑制作用,其EC50分别为60.44、60.78和97.86μg/mL。苦参和丹参提取物以有效质量比为1∶1、1∶3、1∶6时对猕猴桃B.dothidea均表现为增效作用,其CTC分别为133.16、126.16和129.56;而黄岑片·苦参、黄岑片·丹参的提取物复配则均表现为相加作用,其CTC为80.71~117.62。该研究结果可为防治猕猴桃软腐病的药剂组合提供参考。
The virulence of three herbal extracts against the soft rot fungus(Botryosphaeria dothidea) of kiwifruit and the combined effect of the mixture were determined by the mycelial growth rate method.The results showed that Sophora flavescens,Salvia miltiorrhiza and Scutellaria baicalensis showed some inhibitory effects on B.dothidea with EC50of 60.44,60.78 and 97.86 μg/mL,respectively.S.baicalensis and S.flavescens showed potentiation at effective mass ratios of 1∶1,1∶3 and 1∶6,with CTCs of 133.16,126.16 and 129.56,respectively.The extract combinations of S.baicalensis-S.flavescens and S.baicalensis-S.miltiorrhiza all showed additive effects with CTCs ranging from 80.71 to 117.62.The results of this study may provide a reference for determining the combination of agents for the control of soft rot of kiwifruit.
作者
田旭军
廖逊
李荣玉
李明
吴小毛
龙友华
Tian Xujun;Liao Xun;Li Rongyu;Li Ming;Wu Xiaomao;Long Youhua(Institute of Crop Protection/Engineering and Technology Research Center of Kiwifruit,Guizhou University,Guiyang,Guizhou 550025,China)
出处
《山地农业生物学报》
2023年第2期86-89,共4页
Journal of Mountain Agriculture and Biology
基金
农业农村部科技教育司项目(CARS-26-02A)
贵州省优秀青年科技人才资助项目(黔科合平台人才[2021]5614)。
关键词
中药提取物
猕猴桃
葡萄座腔菌
毒力
联合作用
Chinese herbs extracts
kiwifruit
Botryosphaeria dothidea
toxicity
combined effect
