摘要
采用氯磺酸-吡啶法将油茶籽粕多糖(COP)修饰成硫酸酯化油茶籽粕多糖(S-COP),以取代度为指标,研究硫酸酯化多糖的最佳工艺,并对比分析COP和S-COP的降血糖活性。结果表明:最佳工艺为氯磺酸与吡啶体积比1∶4、反应温度60℃、反应时间3 h,最高取代度为1.235,COP与硫酸基成功形成了稳定的硫酸酯化合物;硫酸酯化修饰可明显改善其在水溶液中的溶解性;COP和S-COP对α-葡萄糖苷酶活性均具有良好的抑制作用,抑制能力其质量浓度呈正相关,且S-COP的抑制效果更好。
Camellia oleifera seed meal polysaccharide(COP) was modified into sulfated Camellia oleifera seed meal polysaccharide(S-COP) by chlorosulfonic acid-pyridine method. The best process of sulfated polysaccharide was studied with the degree of substitution as the index, and the hypoglycemic activity of COP and S-COP was compared and analyzed. The results showed that the optimal technology parameters were the volume ratio of chlorosulfonic acid to pyridine 1 ∶4, reaction temperature 60 ℃, reaction time 3 h, and the maximum degree of substitution was 1.235, COP and sulfate group successfully formed stable sulfate compound. Sulfated modification could obviously improve the solubility of COP in aqueous solution. Both COP and S-COP had good inhibitory effect on α-glucosidase activity, and the inhibitory ability was positively correlated with its mass concentration, and the inhibition effect of S-COP was better.
作者
杨建安
张超
文焱炳
方芳
YANG Jian-an;ZHANG Chao;WEN Yan-bing;FANG Fang(Guizhou Research Institute of Chemical Engineering,Guiyang 550002,Guizhou,China;School of Chemistry and Food Engineering,Changsha University of Science and Technology,Changsha 410114,Hunan,China)
出处
《粮食与油脂》
北大核心
2023年第3期133-136,158,共5页
Cereals & Oils
基金
贵州省科技计划项目黔科合支撑([2020]1Y143号)
湖南省教育厅优秀青年项目(19B015)
湖南省重点研发项目(2020SK2100)
贵州省科学技术基金重点项目:黔科合基础([2017]1414)。
关键词
油茶籽粕
多糖
硫酸酯化修饰
降血糖
Camellia oleifera seed meal
polysaccharide
sulfated modification
hypoglycemic
