摘要
α-淀粉酶是一种内切糖苷水解酶,可以水解淀粉等多聚糖内部的α-1,4-糖苷键,生成低聚糖、糊精、麦芽三糖、麦芽糖和少量葡萄糖。由于α-淀粉酶在食品、人体健康监测和制药方面的重要作用,其活性检测广泛应用于工业生产菌株的选育、临床疾病的诊断、糖尿病药物的开发和食品质量的控制中。近年来,随着检测技术的发展,许多更加快速、灵敏的α-淀粉酶检测方法被开发出来。本文综述了近年来α-淀粉酶的检测方法和应用研究进展,分类介绍其检测原理和优缺点,并对未来α-淀粉酶检测方法提出展望,以期为α-淀粉酶检测方法的开发和应用提供参考。
α-amylase is an endonucleoside hydrolase that hydrolyzes the α-1,4-glycosidic bonds inside polysaccharides, such as starch, to generate oligosaccharides, dextrins, maltotriose,maltose and a small amount of glucose. Due to the importance of α-amylase in food industry,human health monitoring and pharmaceuticals, detection of its activity is widely required in the breeding of α-amylase producing strains, in vitro diagnosis, development of diabetes drugs, and the control of food quality. In recent years, many new α-amylase detection methods have been developed with improved speed and sensitivity. This review summarized recent processes in the development and applications of new α-amylase detection methods. The major principle of these detection methods were introduced, and their advantages and disadvantages were compared to facilitate future development and applications of α-amylase detection methods.
作者
张娜英
杨广宇
ZHANG Naying;YANG Guangyu(State Key Laboratory of Microbial Metabolism,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2023年第3期898-911,共14页
Chinese Journal of Biotechnology
基金
国家重点研发计划(2018YFE0200501,2020YFA0907900)
国家自然科学基金(32030063)
天津市合成生物技术创新能力提升行动
上海交通大学特区计划(21TQ1400210)。
关键词
Α-淀粉酶
检测方法
应用
α-amylase
detection methods
applications
