摘要
目的建立基于反渗透技术的水体病毒富集体系。方法以2种灭活病毒疫苗及2种病毒核酸质粒为样本,使用反渗透技术对水中病毒进行富集,并通过实时荧光定量(qRT-PCR)实验对不同材质的反渗透膜、不同孔径及过滤次数的富集效果进行测定与优化。结果富集效率随反渗透膜孔径的降低而提高,0.10μm孔径的反渗透膜对2种待测病毒样本的平均富集效率达到(22.86±0.84)%;尼龙、醋酸纤维素、聚醚砜等3种不同材质的反渗透膜对2种待测病毒样本的富集效率接近;富集效率随水样滤次数的增加而提高,但病毒样品在过滤2次与3次比较富集效率提高不明显。结论0.10μm孔径的醋酸纤维素材质滤膜,在2次过滤的条件下,脊髓灰质炎病毒达到32.64%的病毒富集效率。
Objective To establish a reverse osmosis technology-based method for enrichment of virus in water samples.Methods Two inactivated virus vaccines and two viral nucleic acid plasmids were added into simulated water samples and detected with real-time quantitative reverse transcriptase PCR(qRT-PCR)after reverse osmosis-based enrichment.Reverse osmosis membrane material,membrane pore size,and filtration time were optimized in the enrichments.Results The enrichment efficiency increased with the decrease of pore size of the reverse osmosis membrane.The average virus enrichment efficiency of reverse osmosis membrane with the pore size of 0.10μm was 22.86±0.84%for 2 simulated water samples.The virus enrichment efficiency of nylon,cellulose acetate and polyethersulfone membranes were similar between each other.The enrichment efficiency goes up with the increase of filtration times,but the difference in the efficiency was not significant between two and three times of filtration.Conclusion The efficiency can be up to 32.64%for the enrichment of polio virus in water samples using cellulose acetate filter membrane with 0.10μm aperture and two times of filtration.
作者
刘超
李平
庞燕
赵光远
邬强
LIU Chao;LI Ping;PANG Yan(School of Public Health,Hainan Medical University,Haikou,Hainan Province 571101,China;不详)
出处
《中国公共卫生》
CAS
CSCD
北大核心
2023年第1期98-101,共4页
Chinese Journal of Public Health
基金
海南省自然科学基金(821RC572)
海南省高等学校科学研究项目(Hnky2021-45)。
关键词
环境卫生
水体病毒
反渗透富集浓缩
environment health
virus pollution of water
reverse osmosis enrichment
