摘要
细胞牵引力显微镜方法是测量细胞牵引力的主流工具之一,该方法一般使用荧光微珠作为标志点,通过测量细胞牵引力作用下荧光微珠的位移场,反演得出细胞牵引力场。由于荧光微珠在基底分布的深度不可控,但计算时候默认所有微珠分布在基底表面,一定程度上降低了细胞牵引力场的计算精度。针对荧光微珠深度不可控的问题,提出在弹性基底表面加工图形阵列作为基底变形标志点,开展细胞牵引力测量的方法。在聚二甲基硅氧烷基底表面设计直径3μm,高度0.8μm的凸台阵列作为基底位移标志点。使用有限元仿真软件在图形化基底表面不同位置施加外力,仿真得到凸台阵列的位移场,利用牵引力反演算法计算得到力场,与仿真过程中输入的外力场相比较,结果一致。使用乳鼠心肌细胞在设计加工的图形化弹性基底上开展了牵引力测量实验,并计算得出牵引力场。仿真和实验均表明图形化弹性基底适用于细胞牵引力的测量,为心肌相关疾病病理研究提供了新的研究手段。
Cell traction force microscopy method is one of the mainstream tools for measuring cell traction force, which uses fluorescent microbeads as markers to measure the deformation of the substrate and calculate cell traction force. However, due to the random distribution of fluorescent microbeads in the substrate, the calculation accuracy of the cell traction force field maybe affected. To address the problem of uncontrollable depth of fluorescent microbeads, a method using patterned elastic substrate was proposed to measure cell traction forces, in which the micropattern arrays fabricated on the surface of substrate was used as deformation markers. A micro-pad array with diameter of 3 μm and height of 0.8 μm was designed and fabricated on the polydimethylsiloxane substrate surface as the displacement markers. In the finite element simulation, external forces mimicking cell traction forces was applied at different locations on the substrate surface and the displacements field of the micro-pad array was obtained. Using the displacements field obtained by simulation, the traction force field was calculated through the traction force inversion algorithm, and the results were consistent with the input external forces in the simulation. The patterned elastic substrates were validated by mapping neonatal rat ventricular myocytes contraction forces. Both simulation and experiment demonstrate that the capacity of patterned elastic substrate for generating accurate cell contraction force maps, providing new research method for pathological study of myocardial diseases.
作者
徐红梅
张帆
杜晓晗
樊文强
周贇
陈修寰
赵冠棋
朱疆
Xu Hongmei;Zhang Fan;Du Xiaohan;Fan Wenqiang;Zhou Xun;Chen Xiuhuan;Zhao Guanqi;Zhu Jiang(Key Laboratory of the Ministry of Education for Optoelectronic Measurement Technology and Instrument,Beijing Information Science&Technology University,Beijing 100192,China;Beijing Anzhen Hospital,Capital Medical University,Beijing 100029,China)
出处
《电子技术应用》
2023年第3期30-36,共7页
Application of Electronic Technique
基金
国家自然科学基金(61875237,61975019,61605011,81900454)
北京市教育委员会科技计划重点项目(KZ202011232050)。
关键词
细胞牵引力测量
图形化基底
有限元仿真
牵引力反演
cell traction force mapping
patterned substrates
finite element simulation
traction force inversion
