摘要
采用合理的方法提取角蛋白以进行高值利用是对废弃动物毛发资源化利用的有效途径。基于二硫键断裂机理的不同,采用硫化钠法、L-半胱氨酸法、亚硫酸氢钠法和过氧乙酸法四种方法,从羊毛中提取获得相应的角蛋白,分别命名为K1、K2、K3和K4。对不同提取方法获得的角蛋白进行了提取率、巯基含量、相对分子质量及其分布、亲水性、溶解性以及抗氧化活性等结构和性能的比较。电泳结果显示,角蛋白K1、K2和K3的相对分子质量主要集中在40~60 k Da,而角蛋白K4无明显蛋白条带。K1和K2的游离巯基含量较高,分别为48.3μmol/g和41.2μmol/g,而K4的巯基含量最少,仅为2.0μmol/g。过氧乙酸法提取的角蛋白K4含有较多的磺酸基团,导致其溶解性及亲水性都优于另外三个方法提取的角蛋白。此外,各角蛋白均具有良好的抗氧化活性。以上结果表明,不同提取方法所得的角蛋白在结构与性能上存在较大差异,在生物医药及化妆品领域具有各自的应用潜力。
To effectively recycle waste animal hair from the tanning process, keratin should be extracted by using a reasonable method. In this work, based on the different disulfide bond cleavage mechanisms, the keratin samples were extracted from wool by using four kinds of methods, including sodium sulfide method, L-cysteine method, sodium bisulfite method and peracetic acid method, and assigned as K1, K2, K3, K4, respectively. The structure and properties of the four keratin samples(K1, K2, K3, K4) were investigated and compared, including extraction rate, sulfhydryl content, molecular weight, hydrophilicity, solubility, and antioxidant activity. The electrophoresis results showed that the bands of keratin K1,K2 and K3 were mainly concentrated in the range of 40-60 kDa, while keratin K4 exhibited no obvious protein band. The contents of free sulfhydryl group in K1 and K2 were at 48.3 μmol/g and 41.2 μmol/g, respectively, which were higher than that of K4(2.0 μmol/g). Keratin K4 extracted by peracetic acid method contained more sulfonic acid groups,resulting in higher solubility and hydrophilicity than those extracted by the other three methods. Furthermore, all the extracted keratin samples showed good antioxidant activity. The above results indicated that although the keratin samples obtained by different proposed methods exhibited great difference in their structure and performance, they had application potential in the fields of biomedicine and cosmetics.
作者
邹蝶
王巧娥
程海明
ZOU Die;WANG Qiao’e;CHENG Haiming(National Engineering Laboratory for Clean Technology of Leather Manufacture,Sichuan University,Chengdu 610065,China;Key Laboratory of Leather Chemistry and Engineering of Ministry of Education,Sichuan University,Chengdu 610065,China;Key Laboratory of Cosmetic,China National Light Industry,BeijingTechnology and Business University,Beijing 100048,China)
出处
《皮革科学与工程》
CAS
北大核心
2023年第2期21-27,共7页
Leather Science and Engineering
基金
中国轻工业化妆品重点实验室开放课题(KLC-2021-YB6)。
关键词
羊毛
角蛋白
提取
二硫键
wool
keratin
extraction
disulfide bond
