摘要
目的:观察电针“足三里”及“足三里”加“天枢”对肠易激综合征(IBS)大鼠结肠功能及自主神经平衡性的影响,探讨足三里配伍天枢治疗肠易激综合征是否具有协同作用。方法:Wistar大鼠随机分为对照组、模型组、足三里组、足三里+天枢组,每组14只。采用避水应激法建立IBS模型。足三里组、足三里+天枢组大鼠给予相应穴位的电针治疗,每次20 min,1次/d,连续14 d。采用腹壁回撤反射(AWR)测定大鼠内脏敏感性,PowerLab数据采集分析系统记录大鼠结肠腹直肌肌电、肠电、心电,ELISA法测定大鼠血清环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)的含量,免疫荧光染色法检测大鼠结肠组织中酪氨酸羟化酶(TH)和胆碱乙酰转移酶(ChAT)的阳性表达,Western blot法检测大鼠结肠TH和ChAT蛋白的表达。结果:与对照组比较,模型组大鼠AWR评分升高(P<0.001),心电低频信号(LF)、LF/高频信号(HF),结肠ChAT表达及血清cAMP含量、cGMP含量、cAMP/cGMP均升高(P<0.001,P<0.05),HF、结肠慢波频率、结肠疼痛阈值、结肠TH蛋白表达均降低(P<0.05,P<0.001)。与模型组比较,足三里组、足三里+天枢组大鼠AWR评分均降低(P<0.001),LF、LF/HF、结肠ChAT蛋白表达及血清cAMP、cGMP、cAMP/cGMP含量均降低(P<0.001,P<0.05,P<0.01),结肠慢波频率、结肠疼痛阈值、结肠TH蛋白表达升高(P<0.001,P<0.05)。结论:电针“足三里”及“足三里”加“天枢”对IBS模型大鼠结肠功能、自主神经平衡性均有调整作用,但与单独电针“足三里”比较,电针“足三里”加“天枢”并未显现出明显的协同效应。
Objective To study whether electroacupuncture(EA)of“Zusanli”(ST36)combined with“Tianshu”(ST25)has a synergistic effect in regulating the colonic function and autonomic nerve balance in rats with irritable bowel syndrome(IBS).Methods Male Wistar rats were randomly divided into control,model,EA-ST36,and EA-ST36+ST25 groups,with 14 rats in each group.The IBS model was established by using water avoidance stress method.The visceral hypersensitivity was measured using the abdominal wall retraction reflex(AWR).The rectus abdominis electromyogram(EMG),intestinal electrical activity,and electrocardiogram(ECG)were recorded using a PowerLab data acquisition and analysis system.The contents of serum cAMP and cGMP were determined by ELISA,the expression levels of colonic tyrosine hydroxylase(TH)and choline acetyl-transferase(ChAT)proteins were determined by immunofluorescence staining and Western blot,respectively.Results Compared with the control group,the model group had an evident increase in the levels of AWR,LF,LF/HF,ChAT protein expression,cAMP and cGMP contents and cAMP/cGMP ratio(P<0.001,P<0.05),and a marked decrease in the levels of HF,frequency of slow waves of intestinal EMG,visceral pain threshold(PT),immunoactivity and expression of TH protein(P<0.05,P<0.001).In contrast to the model group,the levels of AWR,LF,LF/HF,ChAT protein expression and immunoactivity,cAMP and cGMP contents and ratio of cAMP/cGMP were significantly reduced(P<0.001,P<0.05,P<0.01),whereas the levels of frequency of slow waves of intestinal EMG,PT,and the immunoactivity and expression of TH were considerably increased(P<0.001,P<0.05)in both EA-ST36 and EA-ST36+ST25 groups.Conclusion EA of both ST36 and ST36+ST25 can relieve visceral pain,and reduce sympathetic activity to improve autonomic nerve balance,but without apparent synergistic effect between EA-ST36 and EA-ST25 in rats with IBS.
作者
王正文
陈碧玮
陈少宗
张丽丽
孙红伟
常晓丽
WANG Zheng-wen;CHEN Bi-wei;CHEN Shao-zong;ZHANG Li-li;SUN Hong-wei;CHANG Xiao-li(Institute of Acupuncture and Moxibustion,Shandong University of Traditional Chinese Medicine,Jinan 250300,China;College of Acupuncture-Moxibustion and Tuina,Shandong University of Traditional Chinese Medicine,Jinan 250355)
出处
《针刺研究》
CAS
CSCD
北大核心
2023年第2期165-171,共7页
Acupuncture Research
基金
国家重点研发计划项目(No.2019YFC1712105)
山东省自然科学基金重大基础研究项目(No.ZR2020ZD17)
山东中医药大学科研创新基金资助(No.2018-220315)。
关键词
肠易激综合征
穴位配伍
电针
结肠功能
自主神经平衡性
Irritable bowel syndrome
Acupoint combination
Electroacupuncture
Colonic function
Autonomic nerve balance
