摘要
目的:观察针刺对偏头痛大鼠三叉神经脊束核(TNC)中星形胶质细胞缝隙连接蛋白43(Cx43)、胶质纤维酸性蛋白(GFAP)、干扰素-γ(IFN-γ)表达水平的影响,探讨针刺治疗偏头痛的可能机制。方法:SD大鼠随机分为空白组、模型组、针刺组、假针刺组,每组11只。采用硝酸甘油颈背皮下注射法(10 mg/kg)制备偏头痛大鼠模型。针刺组针刺双侧“率谷”“阳陵泉”,假针刺组针刺双侧非穴点Ⅰ、非穴点Ⅱ,20 min/次,1次/d,连续9 d。检测大鼠足底机械痛阈值(PWMT)、甩尾潜伏期(TFL)观察疼痛反应;ELISA法检测大鼠TNC中IFN-γ的含量;Western blot法检测大鼠TNC中Cx43和IFN-γ蛋白的表达量;免疫荧光双标法检测大鼠TNC中GFAP、Cx43的共表达情况及神经元核抗原(NeuN)、IFN-γ受体R(IFN-γR)的共表达情况。结果:造模后第3、5、7、9天,与空白组比较,模型组PWMT、TFL明显降低(P<0.01);与模型组和假针刺组比较,针刺组PWMT、TFL明显升高(P<0.01)。与空白组比较,模型组TNC中Cx43蛋白表达水平升高(P<0.01),GFAP与Cx43共表达明显增强(P<0.01),IFN-γ含量和蛋白表达量明显升高(P<0.01),NeuN与IFN-γR共表达明显增强(P<0.01);与模型组和假针刺组比较,针刺组TNC中Cx43蛋白表达水平降低(P<0.01),GFAP与Cx43共表达明显减少(P<0.01),除与假针刺组IFN-γ含量比较外,IFN-γ含量和蛋白表达量明显降低(P<0.05,P<0.01),NeuN与IFN-γR共表达明显减少(P<0.01,P<0.05)。结论:针刺能改善偏头痛疼痛反应,其机制可能是通过抑制TNC中星形胶质细胞Cx43活化,减少促炎因子IFN-γ,发挥抗炎镇痛作用。
Objective To observe the effect of acupuncture on the expression of connexin 43(Cx43),glial fibrillary acidic protein(GFAP),interferon-γ(IFN-γ)in the trigeminal spinal nucleus(TNC)of rats with migraine,so as to explore its mechanisms underlying amelioration of migraine.Methods A total of 44 SD rats were randomly divided into control,model,acu-puncture,and sham acupuncture groups(n=11 in each group).Acupuncture was applied to bilateral“Shuaigu”(GB8)and“Yanglingquan”(GB34)or non-acupointⅠ(the spot about 10 mm superior to the iliac spine and 20 mm lateral to the post-median line)and non-acupointⅡ(behind the iliac spine,the ending-spot of the posterior superior iliac spine at the muscles)on both sides for 20 min,once daily for 9 days.Paw withdrawal latency(mechanical pain threshold,PWMT)and thermal tail flick latency(TFL)were measured using a VonFrey detector and photothermal tail pain meter,respectively.The content of IFN-γof TNC tissue was detected by ELISA.The expression levels of Cx43 and IFN-γproteins of TNC tissue were detected by Western blot.The immunofluorescence dual labeling method was used to detect the positive expression of GFAP and Cx43,IFN-γR and NeuN in TNC tissue,for displaying the activity of Cx43 in astrocytes and IFN-γin neurons,respectively.Results Compared with the control group,both PWMT and TFL at 3,5,7 and 9 days after modeling were significantly decreased(P<0.01),while the expression of Cx43 and IFN-γproteins,the immunofluorescence intensity of GFAP,Cx43,IFN-γR,and the content of IFN-γwere considerably up-regulated in the model group(P<0.01).In comparison with the model group,both PWMT and TFL at 3,5,7 and 9 days after modeling were obviously increased(P<0.01),whereas the expression of Cx43 and IFN-γproteins,the immunofluorescence intensity of GFAP,Cx43,IFN-γR,and the content of IFN-γin the acupuncture group,as well as the protein expression of IFN-γR in the sham acupuncture group were also remarkably decreased(P<0.05,P<0.01).The effect of acupuncture was significan
作者
刘一
周梦荻
郑宇琦
孙铭声
王姿雯
蔡定均
赵凌
LIU Yi;ZHOU Meng-di;ZHENG Yu-qi;SUN Ming-sheng;WANG Zi-wen;CAI Ding-jun;ZHAO Ling(School of Acupunctureand Massage,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China)
出处
《针刺研究》
CAS
CSCD
北大核心
2023年第2期118-124,共7页
Acupuncture Research
基金
国家科学技术部重点研发计划项目(No.2019YFC1709704)
国家自然科学基金面上项目(No.81973962)
国家自然科学基金青年基金项目(No.82004486)
中国博士后科学基金面上项目(No.2020M683643XB)
四川省科技厅科技创新创业苗子工程(No.21MZGC0224)。
