摘要
为探索低温胁迫下幼苗期水稻全基因组DNA甲基化调控机制,通过对3个耐冷性不同的水稻品种进行3~4℃低温处理试验,利用全基因组DNA甲基化测序(WGBS,whole genome bisulfite sequencing)技术分析低温处理后全基因组DNA甲基化水平及模式变化。结果显示:低温处理后,日本晴和9311的胞嘧啶甲基化(mC)都表现下降,而P427的mC值上升。针对启动子区和转录区发生甲基化的基因进行锚定,发现启动子区锚定的基因数远高于转录区,材料P427锚定的基因最多,9311锚定的基因最少。GO、KEGG富集分析发现,低温处理后P427差异甲基化基因主要富集在二萜生物合成、淀粉和蔗糖代谢、苯丙烷类生物合成等代谢通路。结果表明:基因启动子区甲基化对低温胁迫响应基因的调控作用更为重要,甲基化调控基因表达不仅与甲基化程度有关,与甲基化类型也可能存在一定的关系。P427可能通过二萜生物合成、淀粉和蔗糖代谢等代谢通路及激素信号转导通路上的基因影响苗期水稻的耐冷性。本研究进一步加深了对水稻耐冷性响应机制的理解。
In order to explore the regulation mechanism of genome-wide DNA methylation at rice seedlings upon low temperature stress treatment,three rice genotypes showing levels of cold tolerance were treated at 3-4℃,followed by determination of the genome-wide DNA methylation using whole genome bisulfite sequencing(WGBS).By analyzing the distribution of methylation-related genes in the promoter and genic body region of the three samples,it was found that the related genes were mainly distributed in the promoter region.The genotype P427 was found anchoring the highest number of genes,including 9223 genes in the promoter region,and 2906 genes in the body region.The genotype 9311 was found anchoring the fewest genes.GO and KEGG enrichment analysis revealed that the differentially methylated genes were mainly involved in metabolic pathways such as those of diterpene biosynthesis,starch and sucrose metabolism,β-alanine metabolism,and phenylpropane biosynthesis.These results indicated that the methylation in the promoters was important in the regulation of low temperature stress-responsive genes,and the transcriptional regulation was associated with the degree of methylation and the methylation types.The genotype P427 may affect the cold tolerance of rice through modulating the genes in metabolic pathways such as diterpene biosynthesis,starch and sucrose metabolism,and hormone signal transduction pathways.Collectively,these results provided better understanding of the methylation-based response mechanism of cold tolerance in rice.
作者
郭慧
李树杏
甘雨
张宏伟
郝留根
杨占烈
向关伦
GUO Hui;LI Shu-xing;GAN Yu;ZHANG Hong-wei;HAO Liu-gen;YANG Zhan-lie;XIANG Guan-lun(Rice Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang 550006)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2023年第2期376-387,共12页
Journal of Plant Genetic Resources
基金
贵州省科学技术基金项目(黔科合基础[2020]1Z020)
国家自然科学基金(32260525)
贵州省科技计划项目(黔科合支撑[2022]重点028
黔科合支撑[2022]重点025)。
关键词
水稻
DNA甲基化
低温胁迫
差异表达基因
差异甲基化区域
rice
DNA methylation
low temperature stress
differentially expressed gene
differentially methylated region
