摘要
全球超过30%人口患有过敏性疾病,过敏体质者在接触过敏原后会产生IgE抗体,再次接触过敏原后,机体的不同器官会产生速发型过敏反应或迟发型过敏反应。体外检测过敏原特异性IgE是过敏性疾病诊断的重要手段,20世纪80年代以来,分子生物学技术的迅猛发展加速了过敏原分子的鉴定。大量过敏原的鉴定、表征及重组过敏原的生产为分子诊断技术进入临床奠定了坚实基础。近年来,微阵列技术被证实可将过敏原蛋白应用于芯片进行快速的血清学诊断,使用患者少量血清即可得到可靠的检测结果。本文讲述过敏原粗提取物用于过敏性疾病诊断中的不足。重组过敏原的发展、应用促进了组分分辨诊断概念进入临床,介绍了过敏原芯片的发展和应用、过敏原芯片在过敏性疾病中的诊断和应用,并对其将来的发展趋势进行了展望。
Over 30%of the world's population were being affected by allergic diseases.Allergic individuals produce IgE upon allergen exposure.Different organs of the body will produce immediate and late-phase allergic inflammation when being exposure unpon the same allergens.In vitro detection of allergen-specific IgE is an important method for diagnosis of allergic diseases.Since the 1980s,rapid development of molecular biology technology has accelerated the identification of allergen molecules.Identification and characterization of a large number of allergens and production of recombinant allergens have laid a solid foundation for molecular diagnostic technology to enter clinic.In recent years,microarray technology has been proven to be able to apply allergen proteins to chips for rapid serological diagnosis,and reliable test results can be obtained using a small amount of patient serum.This article describes the shortcomings of crude allergen extracts used in the diagnosis of allergic diseases.Development and application of recombinant allergens has promoted concept of molecular resolution diagnosis into clinic,and introduced development and application of allergen chips,diagnosis and application of allergen chips in allergic diseases,and prospects for future development trends.
作者
李青青
崔玉宝(指导)
LI Qingqing;CUI Yubao(Department of Clinical Laboratory,Wuxi People's Hospital Affiliated to Nanjing Medical University,Wuxi 214023,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2023年第2期410-414,420,共6页
Chinese Journal of Immunology
基金
国家自然科学基金项目(NSFC81971511)
江苏省第五期“333工程”科研项目资助计划项目(BRA2017216)
无锡市卫生计生科研重大项目(Z201701)
2020太湖人才计划高端人才项目(2020THRC-GD-7)。
