摘要
目的 探究乙型肝炎病毒(HBV DNA)定量与血清学标志物及血清癌胚抗原(CEA)、血清透明质酸酶(HA)联合检测HBV感染的临床价值。方法 选取2019年1月至2021年11月阜南县人民医院收治的慢性乙型病毒性肝炎患者92例作为研究对象,所有患者均行临床乙肝血清学标志物[包含乙肝表面抗原(HBsAg)、乙肝表面抗体(抗-HBs)、乙肝e抗原(HBeAg)、乙肝e抗体(抗-HBe)、乙肝病毒的核心抗体(抗-HBc)]、CEA、HA以及HBV DNA检测,观察并记录所有患者HBV DNA、CEA、HA的检测结果,比较血清标志物对HBV DNA的检测阳性率,分析HBV DNA定量与血清学标志物及CEA、HA联合检测HBV感染的临床价值。结果 92例患者中,HBV DNA阳性患者54例(58.70%),阴性38例(41.30%)。大三阳组患者HBV DNA阳性31/33例(93.94%),小三阳组患者HBV DNA阳性15/31例(48.39%),其他类型组患者HBV DNA阳性3/28例(10.71%)。92例患者CEA水平4.20(3.20,5.30)ng/mL;HA水平分别为112.00(98.00,126.00)ng/mL。HBV DNA阳性组患者CEA、HA水平显著高于HBV DNA阴性组,差异有统计学意义(P<0.05)。HBV DNA阳性组中,HBsAg阳性检出率显著高于HBeAg,差异有统计学意义(P<0.05)。Spearman相关结果显示,患者的HBV DNA定量与CEA、HBsAg、HA指标成正相关关系(P<0.05)。ROC分析显示:HBV DNA定量与血清学标志物及血清CEA、HA联合检测乙肝病毒感染的AUC=0.985(P<0.05),诊断敏感度为97.76%。结论 HBV DNA定量与血清学标志物CEA、HA联合检测在HBV感染诊断中具有较高的应用价值,可起到互补作用,为临床检测提供了新路径。
Objective To explore the clinical value of HBV DNA quantification,serological markers,CEA and HA in detecting HBV infection. Methods 92 patients with chronic hepatitis B admitted to Funan County People’s Hospital from January 2019 to November 2021 were selected as the study subjects. All patients were tested for clinical hepatitis B serological markers,CEA,HA,and HBV DNA. The positive rate of HBV DNA detection by serum markers was compared,and the clinical value of HBV DNA quantification combined with serological markers,CEA and HA in the detection of HBV infection was analyzed. Results Of the 92 patients,54(58.70%)were HBV DNA positive and 38(41.30%)were negative. 31/33(93.94%)patients in the big three positive group were positive for HBV DNA,15/31(48.39%)patients in the small three positive group were positive for HBV DNA,and 3/28(10.71%)patients in other types of groups were positive for HBV DNA. CEA level in 92 patients was 4.20(3.20,5.30)ng/mL;HA levels were 112.00(98.00126.00)ng/mL. CEA and HA levels in HBV DNA positive group were significantly higher than those in HBV DNA negative group(P<0.05). In the HBV DNA positive group,the positive rate of HBsAg was significantly higher than that of HBeAg(P<0.05). Spearman correlation results showed that the HBV DNA quantity of patients was positively correlated with CEA,HBsAg and HA(P<0.05). ROC analysis showed that AUC of hepatitis B virus infection was 0.985(P<0.05),and the diagnostic sensitivity was 97.76%. Conclusion The combined detection of HBV DNA quantitative and serological markers CEA and HA has high application value in the diagnosis of HBV infection,and can play a complementary role,providing a new path for clinical detection.
作者
郑金萍
高勇
李团团
ZHENG Jinping;GAO Yong;LI Tuantuan(Department of Laboratory Medicine,Funan People's Hospital,Fuyang,Anhui,China,236300;Department of Laboratory Medicine,Fuyang Second People's Hospital,Fuyang,Anhui,China,236300)
出处
《分子诊断与治疗杂志》
2023年第2期222-225,230,共5页
Journal of Molecular Diagnostics and Therapy
基金
安徽省卫生健康委员会科研计划项目(2020SEY015)。
