摘要
目的 通过检测微小RNA 126(miR-126)、微小RNA 381(miR-381)在脓毒症患者血清中的表达,评估二者对脓毒症的预测价值。方法 选取2019年6月至2021年12月189例德阳市人民医院已确诊收治的脓毒症患者,按照严重程度分为79例脓毒症组、65例严重脓毒症组和45例脓毒性休克组,并选取180名健康者作为对照组;受试者工作特征曲线(ROC)分析血清中TNF-α、IL-6、miR-126、miR-381表达水平对脓毒症的预测价值;Logistic回归分析影响脓毒症发生的影响因素。结果 与对照组相比,脓毒症组、严重脓毒症组、脓毒性休克组白细胞计数(WBC)、降钙素原(PCT)、C反应蛋白(CRP)、乳酸(Lac)、高迁移率族蛋白B1(HMGB1)、肿瘤坏死因子α(TNF-α)、白介素-6(IL-6)水平依次显著增加,差异有统计学意义(F=203.191,655.528,565.808,468.356,497.532,97.385,62.398,P<0.05);miR-126、miR-381表达水平在脓毒症组、严重脓毒症组、脓毒性休克组中均显著低于对照组,并随着病情严重程度的增加而显著降低,差异有统计学意义(F=41.349,40.802,P<0.05);多因素分析发现,PCT、CRP、Lac、WBC、HMGB1、TNF-α、IL-6、miR-126和miR-381均是影响脓毒症发生危险因素(P<0.05);miR-126、miR-381联合预测脓毒症发生的AUC显著高于TNF-α、IL-6、miR-126单独预测,差异有统计学意义(Z=1.985,P=0.047;Z=2.121,P=0.034;Z=2.218,P=0.027)。结论 脓毒症患者血清中miR-126、miR-381表达显著降低,二者联合对脓毒症发生具有一定的预测价值。
Objective To detect the expression of microRNA-126(miR-126)and microRNA-381(miR-381)in serum of patients with sepsis,to evaluate the predictive value of the two in sepsis. Methods A total of 189 patients with sepsis admitted to People’s Hospital of Deyang from June 2019 to December 2021were selected. According to the severity,they were divided into 79 cases of sepsis group,65 cases of severe sepsis group and 45 cases of septic shock group,180 healthy people were selected as the control group. The receiver operating characteristic(ROC)curve was used to analyze the predictive value of serum TNF-α,IL-6,miR-126 and miR-381 expression levels for sepsis;Logistic regression analysis was used to analyze the influencing factors of sepsis. Results Compared with the control group,white blood cell count(WBC),procalcitonin(PCT),C-reactive protein(CRP),lactic acid(Lac),and high mobility in the sepsis group,severe sepsis group,and septic shock group Group protein B1(HMGB1),tumor necrosis factor α(TNF-α),interleukin-6(IL-6)levels increased significantly in turn,and the differences were statistically significant(F=203.191,655.528,565.808,468.356,497.532,97.385,62.398,P<0.05). The expression levels of miR-126 and miR-381 in the sepsis group,severe sepsis group and septic shock group were significantly lower than those in the control group,and decreased with the severity of the disease,and the difference was statistically significant(F=41.349,40.802,P<0.05). Multivariate analysis found that PCT,CRP,Lac,WBC,HMGB1,TNF-α,IL-6,miR-126and miR-381 were all risk factors for sepsis(P<0.05). The area under the ROC curve(AUC)of miR-126 and miR-381 combined to predict the occurrence of sepsis was significantly higher than that of TNF-α,IL-6,miR-126alone,and the difference was statistically significant(Z=1.985,P=0.047;Z=2.121,P=0.034;Z=2.218,P=0.027). Conclusion The expressions of miR-126 and miR-381 in the serum of patients with sepsis were significantly decreased,and the combination of the two has a certain predictive value for the oc
作者
林涛
何顶秀
巫金
LIN Tao;HE Dingxiu;WU Jin(Emergency Department of People's Hospital of Deyang City,Deyang,Sichuan,China,618000)
出处
《分子诊断与治疗杂志》
2023年第1期94-98,共5页
Journal of Molecular Diagnostics and Therapy
基金
四川省科技计划项目(2019YFS0443)。
