摘要
目的研究耐甲氧西林金黄色葡萄球菌(MRSA)的标本分布、耐药情况、SCCmec、spa分型、相关毒力因子及生物膜形成情况。方法采用多重PCR扩增SCCmec、spa基因及毒力因子(tst、lukE、pvl、fnbA、fnbB、cna、clfA、clfB);采用Sanger双脱氧链终止法对扩增产物测序;采用微孔结晶紫染色法研究菌株生物膜形成。结果标本来源以分泌物最多,其次是痰;标本分布以儿童病区最多,其次为创伤病区及重症监护病区;SCCmec分型显示I型最多,占56%;spa分型以t437最多,占43%;多数菌株同时携带3种及以上毒力因子,以cna、fnbB和clfA黏附分子为主;60%的MRSA菌株具有较强生物膜形成能力。结论MRSA在儿童病房的流行需要引起注意;ICU病区呼吸机引起的相关感染仍是一大难题。本医疗机构内MRSA(2018—2019年)菌株以t437-SCCmec I型为主要流行克隆株。MRSA携带黏附毒素比率高,容易形成生物被膜,成为治疗的一大难题。
Objective To study the distribution,drug resistance,SCCmec or spa genotype,related virulence factors,and biofilm formations of methicillin-resistant Staphylococcus aureus(MRSA).Methods Multiplex PCR was used to amplify SCCmec or spa genotype.Sanger dideoxy chain termination was used to sequence the amplified products.Bacterial biofilms were studied by the microporous crystal violet staining.Results The most common source of specimens was secretions,followed by sputum.Most of the specimens were distributed in children’s wards,followed by trauma wards and intensive care wards.SCCmec I was the most common type and accounted for 56%in MRSA;t437 was the majority in spa types,and accounted for 43%.Most strains carried three or more virulence factors at the same time,mainly cna,fnbB,and clfA adhesion molecules;60%of MRSA strains had strong biofilm formation ability.Conclusion The prevalence of MRSA in children’s wards needs attention.The related infections caused by ventilator in ICU ward are still a major problem.The t437-SCCmec I strain is the main epidemic clone of MRSA(2018—2019)in our medical institution.MRSA carries a high rate of adhesion toxins and is easy to form biofilms,which has become a problem in treatment.
作者
张丽心
于燕
常育
陈瑞琪
Zhang Li-xin;Yu Yan;Chang Yu;Chen Rui-qi(Department of Clinical Pharmacy,Honghui Hospital,College of Medicine of Xi’an Jiaotong University,Xi’an 710054)
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2023年第1期94-100,共7页
Chinese Journal of Antibiotics
基金
陕西省社会发展基金资助项目(No.2018SF-146)。
关键词
耐甲氧西林金葡菌
SCCMEC
SPA
毒力因子
生物膜
Methicillin-resistant Staphylococcus aureus
SCCmec
spa
Virulence factors
Biofilm formation
