摘要
为探究红壤侵蚀区不同生态恢复林分微生物群落特征,采用高通量测序技术比较阔叶纯林(木荷)、针叶纯林(马尾松)和针阔混交林(木荷林与马尾松林)土壤微生物群落结构和多样性。结果表明:各林分土壤共得到细菌943个分类操作单元(OTU),真菌962个OTU,分类学地位明确的细菌有19门46纲99目138科198属207种,真菌有9门22纲59目109科177属167种。其中,优势细菌门为变形菌门、酸杆菌门和放线菌门,优势真菌门为担子菌门、子囊菌门和被孢菌门。林分类型对土壤真菌丰富度(Chao1指数)和多样性(Shannon指数)影响显著(P<0.05),对土壤细菌丰富度(Chao1指数)和多样性(Shannon和Simpson指数)几乎无影响,针阔混交林土壤真菌丰富度和多样性显著高于纯林土壤(P<0.05)。在门水平上,针阔混交林与阔叶纯林土壤细菌门相对丰度变化差异较小,而与针叶纯林差异较大(P<0.05),针阔混交林和阔叶纯林土壤中放线菌门相对丰度比针叶纯林低9.96%和29.43%。土壤真菌门相对丰度在各林分类型间差异较大,被孢菌门相对丰度在针叶纯林土壤中最高,毛霉门和壶菌门相对丰度在针阔混交林土壤中最高,子囊菌门相对丰度在阔叶纯林土壤中最高。相关分析表明,土壤全氮含量、p H值和速效钾含量是影响土壤细菌群落特征最主要的环境因子。
To explore the microbial community characteristics of different ecological restoration forests in areas with red soil erosion, high-throughput sequencing technology was used to compare the soil microbial community structure and diversity of broad-leaved pure forests(Schima superba), coniferous pure forests(Pinus massoniana), and coniferous and broad-leaved mixed forests(Schima superb forest and Pinus massoniana forest). The results showed that 943 operational taxonomic units(OTUs) of bacteria and 962 OTUs of fungi were obtained from the soil of each stand. We identified 19 phyla, 46 classes, 99 orders, 138 families, 198 genera, and 207 species of bacteria with clear taxonomic status, and 9 phyla, 22 classes, 59 orders, 109 families, 177 genera, and 167 species of fungi. Among them, the dominant bacterial phyla were Proteobacteria, Acidobacter, and Actinomyces, and the dominant fungal phyla were Basidiomycetes, Ascomycetes, and Mortierella. The stand type had a significant impact on the richness and diversity of soil fungi(Chao1 index) and Shannon index(P<0.05) but had little impact on the richness and diversity of soil bacteria(Chao1 index) and diversity(Shannon and Simpson index). The richness and diversity of soil fungi in the coniferous and broadleaved mixed forests were significantly higher than those in the pure forest soil(P<0.05). At the phyla level, the change of relative abundance of soil bacteria in the coniferous and broad-leaved mixed forest and the broad-leaved pure forest has a small difference, but a large difference with the coniferous pure forest(P<0.05). The relative abundance of Actinomycetes in the soil of coniferous and broad-leaved mixed forest and broad-leaved pure forest was 9.96% and 29.43% lower than that of coniferous pure forest. The relative abundance of soil Mycophyta varied greatly among different forest classification types. The relative abundance of Mortierella was the highest in coniferous pure forest soil, that of Mucor and Chrysophyta was the highest in coniferous and broad-lea
作者
管鸿智
黄荣珍
朱丽琴
王金平
姬绍晖
林丽靖
GUAN Hongzhi;HUANG Rongzhen;ZHU Liqin;WANG Jinping;JI Shaohui;LIN Lijing(School of Hydraulic&Ecological Engineering,Nanchang Institute of Technology,Nanchang,Jiangxi 330099,China;Jiangxi Key Laboratory for Restoration of Degraded Ecosystems&Watershed Ecohydrology,Nanchang,Jiangxi 330099,China)
出处
《森林与环境学报》
CSCD
北大核心
2023年第2期177-184,共8页
Journal of Forest and Environment
基金
国家自然科学基金项目“外源有机碳对红壤退化地微团聚体形成与稳定的影响机制”(31660192)
江西省教育厅项目“凋落物、细根和菌根输入对退化红壤微生物群落结构的影响”(GJJ190974)
江西省教育厅科学技术研究项目“马尾松林灾毁迹地水土流失阻控技术研究”(GJJ211901)。
关键词
土壤微生物群落特征
生态恢复林分
红壤区
高通量测序
characteristics of soil microbial community
ecological restoration stand
red soil area
high throughput sequencing
