摘要
【目的】建立鳗鱼(Muraenesox cinereus)纳米粒子运载体系,探究其对HepG2人肝癌细胞氧化损伤的保护作用。【方法】制备鳗鱼纳米粒子-花色苷复合物(ENPs-C3G)运载体系,并测定其ABTS+清除能力以及FRAP还原力,利用H2O2建立HepG2氧化应激模型,并探究ENPs-C3G对H2O2诱导的HepG2细胞内活性氧(ROS)、NO质量摩尔浓度以及超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力水平的影响。【结果】ENPs-C3G复合物对ABTS+清除能力显著高于CYD-3-G(P<0.05),复合物质量浓度比为1∶20组ENPs-C3G的ABTS+清除能力最强,接近100%;ENPs-C3G对亚铁离子还原力高于CYD-3-G,1∶40组ENPs-C3G亚铁离子还原力为(0.400±0.033)μmol/g,显著高于CYD-3-G对亚铁离子还原力(0.340±0.033)μmol/g(P<0.05);H2O2浓度为200μmol/L,处理6 h时,细胞存活率相较于空白组显著降低(P<0.05);ENPs-C3G复合物对H2O2诱导氧化损伤的HepG2细胞内ROS水平和NO质量摩尔浓度相较于HepG2氧化损伤模型均表现出显著降低现象(P<0.05),CAT和SOD酶活力水平相较于HepG2氧化损伤模型均表现为显著提高(P<0.05)。【结论】鳗鱼纳米粒子-花色苷复合物对H2O2诱导的HepG2细胞氧化损伤具有一定的保护作用。
【Objective】To explore the protective effect of eel(Muraenesox cinereus)nanoparticles on oxidative damage of HepG2 from human liver cancer cells,and establish the eel nanoparticles-anthocyanin delivery system.【Methods】Eel nanoparticle-anthocyanin complex(ENPs-C3G)delivery system was conducted to evaluate ABTS+radical scavenging activity and the ability to reduce FRAP.Furthermore,the HepG2 oxidative stress model was established using H2O2,the effects of the ENPs C3G complex on the H2O2-induced ROS,NO content,and SOD,as well as CAT activity in HepG2 cells were determined.【Results】The ABTS+radical scavenging activity of ENPS-C3G complex was significantly higher than that of CYD-3-G(P<0.05)and ENPS-C3G complex in 1∶20 group(material mass ratio)showed the strongest effect on the scavenging ABTS+·radicals,close to 100%.The FRAP reduction effect of ENPS-C3G was also stronger than that of CYD-3-G.The FRAP reduction effect of ENPS-C3G was(0.400±0.033)μmol/g in 1∶40 group,which was significantly higher than that of CYD-3-G with(0.340±0.033)μmol/g(P<0.05).Cell culture study revealed that when H_(2)O_(2)(concentration at 200μmol/L)treated HepG2 cells for 6 h,the cell viability was significantly decreased compared with the blank group(P<0.05).The levels of ROS and the content of NO in HepG2 cells induced by H_(2)O_(2)were significantly reduced by ENPS-C3G complex compared with oxidative damage model(P<0.05),the enzyme activities of CAT and SOD were significantly increased(P<0.05).【Conclusion】Eel nanoparticle-anthocyanin complex has a promising antioxidant activity in vitro and protective effect on H2O2-induced oxidative damage in HepG2 cells.
作者
滕慧
吴婷
张畅
陈雷
TENG Hui;WU Ting;ZHANG Chang;CHEN Lei(College of Food Science and Technology,Guangdong Ocean University/Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety/Guangdong Province Engineering Laboratory for Marine Biological Products/Guangdong Provincial Engineering Technology Research Center of Seafood/Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Zhanjiang 524088,China;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Key Technologies of Marine Food Deep Processing:Collaborative Innovation Center Jointly Constructed by the Ministry and the Province,Dalian University of Technology,Dalian 116034,China)
出处
《广东海洋大学学报》
CAS
CSCD
北大核心
2023年第1期103-110,共8页
Journal of Guangdong Ocean University
基金
国家自然科学基金面上项目(32072209)。
关键词
鳗鱼
纳米粒子
花色苷
氧化应激
保护作用
eel
nanoparticles
anthocyanins
oxidative damage
protective effect
