摘要
【目的】分析镉(Cd)胁迫条件下钻叶紫菀(Aster subulatus Michx.)的生理指标变化情况及对Cd的富集特征,为挖掘重金属富集植物及推动利用植物修复Cd污染环境提供参考依据。【方法】开展不同Cd浓度[0(对照,CK)及2.5、5.0、10.0、15.0、20.0和30.0 mg/L)]胁迫钻叶紫菀的营养液砂培试验,测定不同浓度Cd处理钻叶紫菀的株高、生物量(干重)、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性、叶绿素含量及Cd吸收累积量,分析各处理钻叶紫菀受Cd胁迫后的生理调节能力及对Cd的富集转运能力。【结果】随着Cd处理浓度的增大及培养时间的延长,钻叶紫菀的株高和生物量均较CK出现一定程度降低,而叶片CAT和POD活性及叶绿素含量均呈先上升后下降再上升的变化趋势,但最终均高于CK;除2.5 mg/L Cd处理外,其他浓度Cd处理钻叶紫菀体内的Cd含量均表现为根>叶>茎,说明钻叶紫菀根、茎和叶的Cd吸收累积能力总体上均随着Cd胁迫浓度的增加呈逐渐增大变化特征;随着Cd胁迫浓度的增加,钻叶紫菀叶部的Cd转运系数和富集系数均呈先增大后减小再增大的变化趋势,茎部的Cd转运系数和富集系数均逐渐减小,而根部的Cd富集系数呈先减小后增大再减小的变化趋势。在30.0 mg/L Cd胁迫时,钻叶紫菀根、茎和叶的Cd含量最高,分别达1075.13、34.33和179.47 mg/kg,此时叶部的Cd含量超过100.00 mg/kg,呈现典型的Cd超富集植物特征。【结论】钻叶紫菀可通过调节Cd在其体内的富集和转运来适应Cd胁迫生境,对Cd胁迫具有较强的耐受性和富集能力。其中,在2.5~30.0 mg/L Cd胁迫条件下,钻叶紫菀的生理指标受影响较小,可正常生长,因此,可尝试将钻叶紫菀作为Cd富集或超富集能力植物用于修复Cd污染土壤或湿地。
【Objective】The physiological indexes and enrichment characteristics of Aster subulatus Michx.(AsM)under cadmium(Cd)stress were studied,so as to provide a reference basis for finding heavy metal enrichment plants and promoting the use of plants to repair Cd polluted environment.【Method】The nutrient solution sand culture test under different Cd concentration[0(Control,CK)and 2.5,5.0,10.0,15.0,20 and 30.0 mg/L]was carried out,and the plant height,biomass(dry weight),peroxidase(CAT)activity,peroxidase(POD)activity,chlorophyll content and Cd absorption accumulation of AsM were measured,and then the physiological regulation ability,Cd accumulationt and translocation ability of AsM were analyzed under different Cd stress treatments.【Result】With the increase of Cd concentration and culture time,the plant height and biomass of AsM decreased somehow compared to CK,POD activity,and CAT activity and chlorophyll content in leaves increased initially but decreased then increased again,but they were higher than CK;Cd content in AsM showed roots>leaves>stems except for in 2.5 mg/L Cd treatment,and the results showed that Cd absorption and accumulation capacity of roots,stems and leaves increased gradually,as Cd stress concentration increased;The Cd translocation factor and bioconcentration factor in leaves showed a trend of increasing initially but decreasing then increasing again,the Cd translocation factor and bioconcentration factor in stems gradually decreased,and the Cd bioconcentration factor in roots showed a trend of decreasing initially but increasing then decreasing again,as Cd stress concentration increased.The content of Cd in roots,stems and leaves were the highest,reaching 1075.13,34.33 and 179.47 mg/kg respectively,for 30.0 mg/L Cd stress treatment.At this time,the content of Cd in leaves exceeded 100.00 mg/kg,showing a typical Cd hyperaccumulation plant standard.【Conclusion】Cd has little impact on the physiology of AsM,and they can grow normally,show strong tolerance and accumulation ability to Cd
作者
陈伟
蒋文艳
杨玉霞
廖洁
梁雪莲
王海军
魏源文
王天顺
CHEN Wei;JIANG Wen-yan;YANG Yu-xia;LIAO Jie;LIANG Xue-lian;WANG Hai-jun;WEI Yuan-wen;WANG Tian-shun(Research Institute of Agro-products Quality Safety and Testing Technology,Guangxi Academy of Agriculture Sciences/Total Quality Control Technology Nanning Center for National New&Special Agricultural Product,Nanning 530007,China)
出处
《西南农业学报》
CSCD
北大核心
2022年第12期2860-2866,共7页
Southwest China Journal of Agricultural Sciences
基金
广西自然科学基金项目(2020GXNSFAA259051,2021GXNSFBA196028)
广西农业科学院科技发展基金项目(桂农科2021JM30,桂农科2021JM117,桂农科2021YT137)。
关键词
钻叶紫菀
镉胁迫
富集系数
转运系数
植物修复
Aster subulatus Michx.(AsM)
Cadmium stress
Enrichment coefficient
Translocation coefficient
Phytoremediation
