摘要
目的 通过观察HBeAg不同状态下慢性乙型肝炎(chronic hepatitis B,CHB)的HBV RNA表达水平、检出率分析以及与HBV DNA、HBsAg的相关性,探索血清HBV RNA在CHB管理中的价值。方法 本研究共纳入2021年1月—12月在杭州市西溪医院门诊及住院部就诊的CHB患者253例,包括HBeAg阳性的患者103例和HBeAg的阴性患者150例,检测血清HBV RNA定量,并分析其与HBsAg、HBV DNA等的相关性。结果 在HBeAg阳性组与HBeAg阴性组之间,HBV RNA及HBV DNA的表达水平,差异均有统计学意义(P<0.001)。HBeAg阳性患者的HBV RNA、HBV DNA、HBsAg、ALT、AST定量均高于HBeAg阴性患者。HBeAg阳性组中,血清HBV RNA与HBV DNA、HBsAg成正相关(r值分别为0.855、0.669,P<0.001);HBeAg阴性组中,血清HBV RNA与HBV DNA、HBsAg成正相关(r值分别为0.476、0.375,P<0.001)。在CHB未治患者中HBV RNA与HBsAg、HBV DNA成正相关(r值分别为0.779、0.918,P<0.001)。CHB经治患者中,HBV RNA与HBsAg成正相关(r=0.306,P<0.001)。结论 血清HBV RNA与HBsAg、HBV DNA具有相关性,这种相关性在HBeAg阳性患者中要优于HBeAg阴性患者,慢性乙型肝炎未治患者中要优于慢性乙型肝炎经治患者;HBeAg阳性患者的病毒水平及肝功能异常率更高。
Objective The value of serum HBV RNA in chronic hepatitis B(CHB) management was explored by observing the expression level and detection rate of HBV RNA in CHB under different states of HBeAg and the correlation with HBV DNA and HBsAg. Methods A total of 253 CHB patients, including 103 HBeAg-positive patients and 150 HBeAg-negative patients, who were admitted to the outpatient and inpatient departments of Hangzhou Xixi Hospital from January 2021 to December 2021, were included in this study. Serum HBV RNA quantification was detected and its correlation with HBsAg and HBV DNA was analyzed. Results The level differences of HBV RNA and HBV DNA were statistically significant between HBeAg-positive group and HBeAg-negative group(P<0.001). The quantifications of HBV RNA, HBV DNA, HBsAg, ALT and AST in HBeAg-positive patients were higher than those in HBeAg-negative patients. In HBeAg positive group, serum HBV RNA was positively correlated with HBV DNA and HBsAg(r=0.855, r=0.669, P<0.001). In HBeAg negative group, serum HBV RNA was correlated with HBV DNA and HBsAg(r=0.476, r=0.375, P<0.001), but not as positively as HBeAg positive group. HBV RNA was positively correlated with HBsAg and HBV DNA in patients without CHB treatment(r=0.779, r=0.918, P<0.001). HBV RNA was positively correlated with HBsAg in CHB treated patients(r=0.306, P<0.001). Conclusion Serum HBV RNA is significantly correlated with HBsAg and HBV DNA, which is better in HBeAg-positive patients than that in HBeAg-negative patients, and better in untreated patients than that in treated patients. HBeAg-positive patients had higher viral levels and abnormal rate of liver function.
作者
张晓晶
武瑞
黄伟
刘雁
吴晓瑛
刘寿荣
ZHANG Xiao-jing;WU Rui;HUANG Wei;LIU Yan;WU Xiao-ying;LIU Shou-rong(Department of Liver Disease,Hangzhou Xixi Hospital(Hangzhou Sixth People's Hospital),Zhejiang 310023,China)
出处
《中国卫生检验杂志》
CAS
2023年第3期257-260,共4页
Chinese Journal of Health Laboratory Technology
基金
国家“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项“十三五”计划(2017ZX10302201)
国家科技重点研发专项(2018YFC1705700)
杭州市科技计划引导项目(20201231Y040)。
