摘要
目的 探讨不同检验方法检测生殖道沙眼衣原体感染结果准确率的临床应用。方法 对106例疑似生殖道沙眼衣原体感染的患者尿道、宫颈拭子和尿液分别用酶联免疫吸附试验(ELISA)、抗原快速检测试验(免疫层析试验)和生殖道沙眼衣原体核酸检测(PCR),比较不同检验方法诊断阳性率、灵敏度和特异度。结果 以生殖道沙眼衣原体核酸检测为“诊断标准”,其阳性率为97.17%(103/106),酶联免疫吸附试验阳性率为84.91%(90/106),免疫层析试验阳性率为80.19%(85/106),生殖道沙眼衣原体核酸检测试验的灵敏度和特异度高于酶联免疫吸附试验和免疫层析试验(P<0.01);酶联免疫吸附试验的灵敏度为87.25%,特异度为75.00%;免疫层析试验的灵敏度为82.18%,特异度为60.00%;酶联免疫吸附试验特异度高于免疫层析试验(P<0.05)。结论 根据临床诊断和治疗的不同需要,选择生殖道沙眼衣原体感染适宜的检测方法,可以提高临床诊断的准确性,避免误诊。
Objective This paper aims to compare the accuracy of different methods in the detection of Chlamydia trachomatis(Ct) infection and explore the clinical application. Methods Urinary tract, cervical swab and urine samples in 106 cases of suspected genital tract Ct infection were detected by using enzyme-linked immunosorbent assay(ELISA), and antigen rapid test(immunochromatography) and genital tract Ct nucleic acid testing(PCR) test, and their diagnostic positive rates, sensitivities and specificities were compared. Results Using the PCR test of genital tract Ct as diagnostic criteria, the positive rate was 97.17%(103/106), 84.91%(90/106) by ELISA and 80.19%(85/106) by immunochromatography. The sensitivity and specificity of PCR were higher than those of ELISA and immunochromatography(P<0.01). The sensitivity and specificity of ELISA were 87.25% and 75.00% respectively. The sensitivity and specificity of immunochromatography were 82.18% and 60.00% respectively. The specificity of ELISA was higher than that of immunochromatography(P<0.05). Conclusion According to different needs of clinical diagnosis and treatment, selection of suitable detection methods for genital CT infection can improve the accuracy of clinical diagnosis and avoid misdiagnosis.
作者
曹守勤
杨丽娟
韩磊
CAO Shou-qin;YANG Li-juan;HAN Lei(Ningxia Provincial Center of Disease and Prevertion,Yinchuan,Ningxia 750004,China;不详)
出处
《中国卫生检验杂志》
CAS
2023年第2期222-223,227,共3页
Chinese Journal of Health Laboratory Technology
关键词
生殖道沙眼衣原体感染
核酸检测
酶联免疫吸附试验
免疫层析试验
临床应用
Chlamydia trachomatis
Nucleic acid detection
Enzyme-linked immunosorbent assay
Immunochromatographic assay
Clinical application
