摘要
目的 分析广西崇左市龙州县HIV/AIDS患者中HIV-1分子传播网络特征,并探讨网络形成的相关影响因素。方法 对2003-2021年龙州县报告的HIV/AIDS患者采集血样,通过DNA提取、pol区扩增、测序及序列整理,使用HIV-TRACE筛选最佳基因距离及构建分子传播网络。对分类资料进行χ^(2)检验,使用Logistic回归模型分析入网和高连接度个体的影响因素。结果 共收集到545份研究样本,成功获得361例HIV/AIDS患者的HIV-1 pol区序列。HIV亚型以CRF01_AE(88.09%)为主,其次为CRF07_BC(5.26%)和CRF08_BC(3.88%)。多因素结果显示,无性病史(OR=3.238,95%CI:1.134~10.642)、已婚/同居(OR=1.953,95%CI:1.03~3.758)的患者更容易入网;年龄≥50岁(OR=2.656,95%CI:1.394~5.109)的患者更容易成为该网络的高连接度个体,而高中/中专及以上(OR=0.251,95%CI:0.057~0.784)更不容易成为高连接度个体。结论 2003-2021年,龙州县艾滋病传播的关键人群为年龄≥50岁及低文化程度的患者,当地的相关部门应加强对该人群的治疗和管理及靶向干预,以减少HIV的二代传播。
Objective To analyze the characteristics of the HIV-1 molecular transmission network among people living with HIV/AIDS(PLWHA) in Longzhou County, Guangxi and explore the associated factors of transmission networks. Methods Blood samples were collected from PLWHA in Longzhou County from 2003 to 2021, through DNA extraction, HIV-1 pol amplification and sequencing. We used HIV-TRACE to screen the optimal gene distance of molecular transmission networks and to form transmission networks. A chi-square test and multiple logistic regression models were used to analyze the associated factors of transmission cluster and high-degree individuals. Results A total of 545 blood samples were collected and the HIV-1 pol sequences of 361 HIV/AIDS patients were obtained by amplification and sequencing. The main subtype of HIV was CRF01_AE(88.09%), followed by CRF07_BC(5.26%) and CRF08_BC(3.88%). In a multivariate analysis, patients who were without a history of sexually transmitted diseases(OR=3.238,95%CI: 1.134-10.642) and married or cohabiting(OR=1.953,95%CI: 1.03-3.758)were more likely to be members of transmission cluster. Patients aged 50 years or more(OR=2.656,95%CI: 1.394-5.109) were more likely to be high-degree individuals in the clusters. Patients who were with higher education(OR=0.251,95%CI: 0.057-0.784) were less likely to be high-degree individuals in the clusters. Conclusions From 2003 to 2021, PLWHA aged ≥50 years and with low education levels were the key population of HIV-1 transmission in Longzhou County. The local department should strengthen treatment and management interventions for the key population to reduce the secondary transmission of HIV.
作者
覃彩
黄智冰
黄争魁
何洋
黄丽冬
张谊
钟姗妹
李牧
罗通
张容静
叶力
梁冰玉
QIN Cai;HUANG Zhibing;HUANG Zhengkui;HE Yang;HUANG Lidong;ZHANG Yi;ZHONG Shanmei;LI Mu;LUO Tong;ZHANG Rongjing;YE Li;LIANG Bingyu(Public health department of Guangxi Medical University&Guangxi key laboratory for AIDS prevention and treatment,Nanning 530021,China;Longzhou Center for Disease Control and Prevention,Longzhou532400)
出处
《中国艾滋病性病》
CAS
CSCD
北大核心
2023年第2期136-141,共6页
Chinese Journal of Aids & STD
基金
广西科技计划项目资金(桂科AB19245038)
国家自然科学基金(82103899,82060610)。
