摘要
目的探讨龟苓膏对温病湿热证模型大鼠体温、胃肠运动、胃肠激素、Th1/Th2细胞因子及水液代谢的影响。方法将60只SD大鼠按随机数字表法分为空白组、模型组、莫沙必利组及龟苓膏低(3.4 g/kg)、中(6.8 g/kg)、高剂量组(13.6 g/kg),每组10只。除空白组外,其余各组采用“环境因素+肥甘饮食+生物因子”方法制备温病湿热证大鼠模型,造模后连续灌胃相应药物7 d,观察给药期间大鼠一般状态、体重及体温,采用称重法计算大鼠胃残留率,炭末推进法计算大鼠肠推进率,采用ELISA法检测大鼠血清胃动素(MTL)、胃泌素(GAS)、生长抑素(SS)、P-物质(SP)、IL-4和干扰素-γ(IFN-γ)及电解质水平,采用Real-time PCR法检测大鼠结肠组织水通道蛋白3(AQP3)mRNA水平。结果造模后第22天,与模型组比较,龟苓膏高剂量组大鼠体重升高(P<0.05);龟苓膏中、高剂量组大鼠第19~20天肛温降低(P<0.01);龟苓膏中、高剂量组大鼠胃排空率、小肠推进率增加(P<0.01或P<0.05),血清MTL、GAS、SP水平升高(P<0.01或P<0.05),SS水平降低(P<0.01或P<0.05);IL-4、IFN-γ水平及IFN-γ/IL-4比值降低(P<0.01);龟苓膏高剂量组AQP3 mRNA[(1.16±0.25)比(0.23±0.01)]水平上调(P<0.01)。结论龟苓膏可有效改善因复合因素所致的温病湿热证模型大鼠的活动状态,其作用机制可能与增强胃肠运动、增加胃肠激素分泌、恢复免疫系统Th1/Th2动态平衡及促进水分从肠腔转运有关。
Objective To investigate the effects of Guiling Gao on body temperature,gastrointestinal motility,gastrointestinal hormones,Th1/Th2 cytokines and water metabolism in rats with damp-heat syndrome.Methods Totally 60 SD rats were randomly divided into control group,model group,mosapride group,Guiling Gao low dose group(3.4 g/kg),medium dose group(6.8 g/kg)and high dose group(13.6 g/kg)according to random number table method,with 10 rats in each group.Except for the blank group,the other groups adopted the method of"environmental factors+fat and sweet diet+biological factors"to prepare the rat model of damp heat syndrome of febrile diseases.After modeling,they were administered by gavage for 7 days.During the experiment,the general state,body weight and body temperature were observed,the gastric residue rate of rats was calculated by weighing method,the intestinal propulsion rate of rats was calculated by charcoal propulsion method,and the levels of serum motilin(MTL),gastrin(GAS),somatostatin(SS),substance P(SP),IL-4 and interferon-γ(IFN-γ)were detected by ELISA,and the changes of aquaporin 3(AQP3)mRNA transcription level were detected by real-time PCR.Results Compared with the model group,the weight of rats in Guiling Gao high dose group increased after experiment of 22 days(P<0.05),and body temperature of rats in Guiling Gao medium and high dose group decreased in 19-20 day(P<0.01);and the gastric emptying rate and the small intestine propulsion rate of small intestine in Guiling Gao medium and high dose group increased significantly(P<0.01 or P<0.05);the serum MTL,GAS and SP levels increased(P<0.01 or P<0.05),and SS decreased(P<0.01 or P<0.05)in the Guiling Gao medium and high dose groups;The levels of IL-4,IFN-γand IFN-γ/IL-4 ratio decreased(P<0.01);The expression of AQP3 mRNA(1.16±0.25 vs.0.23±0.01)in the Guiling Gao high dose group was up-regulated(P<0.01).Conclusions Guiling Gao can effectively improve the activity state of damp-heat syndrome model rats caused by complex factors.This mechanism may be re
作者
宋玲
李晗
高云航
陈腾飞
侯红平
彭博
张广平
叶祖光
Song Ling;Li Han;Gao Yunhang;Chen Tengfei;Hou Hongping;Peng Bo;Zhang Guangping;Ye Zuguang(Aerosol Inhalation Preparation Research Center,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《国际中医中药杂志》
2023年第1期47-53,共7页
International Journal of Traditional Chinese Medicine
基金
国家重点研发计划项目(2018YFC1602104)。
关键词
龟苓膏
温病
湿热
胃肠活动
胃肠激素
水液代谢
大鼠
Gui ling concentrated decoction
Seasonal prevalent diseases
Dampness heat
Gastrointestinal motility
Gastrointestinal hormones
Water metabolism
Rats
