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超声波协同离子液体预处理对酒糟纤维素酶解的影响

Effects of Ultrasonic Combined with Ionic Liquid Pretreatment on the Enzymolysis of Cellulose Derived from Distiller’s Grains
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摘要 酿酒酒糟由于富含纤维素而导致其在生物体内的利用率低,试验探究了超声波协同离子液体预处理对酒糟纤维素酶解效果的影响。根据前期的研究结果,采用单因素试验和正交试验对超声波工作条件进行参数优化,得出最优工艺条件:超声波功率密度333 W/L、超声波处理温度55℃、超声波处理时间15 min、超声波工作间歇比5∶2 (s/s)。经此条件处理,酒糟纤维素酶解转化率能达到12.05%,与单一超声波处理、单一离子液体预处理和不做任何处理的相比,转化率分别提高114.4%, 11.2%和190.4%。试验结果可为酒糟纤维素的高值化利用提供新方法。 The utilization of distiller’s grains is very low in vivo due to its abundant content of cellulose. The effects of ultrasonic combined with ionic liquid pretreatment on the enzymolysis of distiller’s grains cellulose were studied in order to improve the utilization of cellulose in the present study. Based on our previous results, the effects of ultrasonic pretreatment conditions on the enzymatic hydrolysis of distiller’s grains cellulose was optimized by single-factor experiments and orthogonal experiments. The results showed that the optimum ultrasound pretreatment condition was ultrasonic power density of 333 W/L,temperature of 55 ℃, time of 15 min and intermittent ratio of 5︰2(s/s), under which the conversion rate of distiller’s grains cellulose was 12.05%. Compared with the ultrasound treated, the ionic liquid treated and the untreated samples under the same enzymatic hydrolysis conditions, the conversion rate increased by 114.4%, 11.2% and 190.4%, respectively. The results indicated that the ultrasonic combined with ionic liquid pretreatment was hopefully a new method to utilization of distiller’s grains cellulose.
作者 雷玉立 曾凯 龙艳 范春泉 金建 陈文浩 LEI Yuli;ZENG Kai;LONG Yan;FAN Chunquan;JIN Jian;CHEN Wenhao(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621000;Solid-State Fermentation Resource Utilization Key Laboratory of Sichuan Province,Yibin 644000;School of Economics and Management,Southwest University of Science and Technology,Mianyang 621000)
出处 《食品工业》 CAS 2023年第1期22-25,共4页 The Food Industry
基金 固态发酵资源利用四川省重点实验室开放基金项目(2018GTY007) 四川省大学生创新创业训练项目(S202210619101)。
关键词 离子液体 超声波 酒糟 纤维素 酶解 ionic liquids ultrasound distiller’s grains cellulose enzymolysis
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