摘要
目的 评价萝卜硫素(SFN)对力竭运动所致小鼠骨骼肌损伤和疲劳的影响。方法 将60只雄性昆明小鼠随机分成对照组(Control)、力竭运动组(EE)、SFN低剂量组(SFN-L,20 mg/kg/d)、SFN中剂量组(SFN-M,40mg/kg/d)和SFN高剂量组(SFN-H,60 mg/kg/d),每组12只。共给药4周,然后进行力竭运动。测定各组小鼠肌酸激酶(CK)、乳酸(LA)、乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)水平。苏木精-伊红染色观察组织病理学改变。透射电镜观察线粒体超微结构。通过测定柠檬酸合成酶(CS)、琥珀酸脱氢酶(SDH)及肝脏和股四头肌糖原含量来评估能量水平。用RT-qPCR和Western blot检测骨骼肌中与线粒体自噬相关的mRNA和蛋白表达水平。结果 与EE组相比,SFN-L组、SFN-M组和SFN-H组的CK、LA、LDH和MDA水平显著降低,而SOD和CAT活性显著升高(P<0.05)。SFN明显减轻了EE小鼠的骨骼肌损伤并改善了线粒体形态。与EE组相比,SFN-L组、SFN-M组和SFN-H组的肝糖原和肌糖原含量、CS表达和SDH活性显著升高(P<0.05)。与EE组相比,SFN-L组、SFN-M组和SFN-H组小鼠股四头肌组织中PINK1、Parkin、Lc3-Ⅱ、p62和ubiquitin的蛋白表达水平显著降低(P<0.05)。与EE组相比,SFN-L组、SFN-M组和SFN-H组小鼠股四头肌组织中PINK1和Parkin的mRNA表达水平显著降低(P<0.05)。结论 SFN通过抑制PINK1/Parkin信号通路介导的线粒体自噬来发挥抗疲劳作用。
Objective To evaluate the effect of sulforaphane(SFN) on skeletal muscle damage and fatigue in mice induced by exhaustive exercise(EE).Methods Sixty male Kunming mice were randomly divided into control group(Control),exhaustive exercise group(EE),SFN low-dose group(SFN-L,20 mg/kg/d),SFN medium-dose group(SFN-M,40 mg/kg/d) and SFN high-dose group(SFN-H,60 mg/kg/d),each group was 12 animals.After total of 4 weeks of administration,the exhaustive exercise was done.The levels of creatine kinase(CK),lactate(LA),lactate dehydrogenase(LDH),malondialdehyde(MDA),superoxide dismutase(SOD) and catalase(CAT) in each group were determined.Histopathological changes were observed by hematoxylin-eosin staining.The ultrastructure of mitochondria was observed by transmission electron microscope.Energy levels were assessed by measuring citrate synthase(CS),succinate dehydrogenase(SDH),and liver and quadriceps glycogen content.RT-qPCR and Western blot were used to detect mRNA and protein expression levels related to mitochondrial autophagy in skeletal muscle.Results Compared with the EE group,the levels of CK,LA,LDH and MDA in the SFN-L,SFN-M and SFN-H groups reduced significantly,while the SOD and CAT activities increased significantly(P<0.05).SFN significantly reduced skeletal muscle damage in EE mice and mitochondrial morphology was improved.Compared with the EE group,the liver glycogen and muscle glycogen content,CS expression and SDH activity in the SFN-L,SFN-M and SFN-H groups increased significantly(P<0.05).Compared with the EE group,the protein expression levels of PINK1,Parkin,Lc3-Ⅱ,p62 and ubiquitin in the quadriceps femoris tissue of the SFN-L,SFN-M and SFN-H groups reduced significantly(P<0.05).Compared with the EE group,the mRNA expression levels of PINK1 and Parkin in the quadriceps femoris tissues of mice in the SFN-L,SFN-M and SFN-H groups reduced significantly(P<0.05).Conclusion SFN exerts anti-fatigue effects through inhibiting mitochondrial autophagy mediated by PINK1/Parkin signaling pathway.
作者
国春鼎
杨军霞
李鹏程
GUO Chunding;YANG Junxia;LI Pengcheng(Bozhou University,Anhui Bozhou 236800,China;Physical Education College of Huainan NormalUniversity,Anhui Huainan 232001,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2022年第6期1158-1165,共8页
Chinese Journal of Food Hygiene
