摘要
目的 探讨肝郁脾虚型消化不良(FD)的发病机制及平胃胶囊促胃肠动力的干预机制。方法 将48只♂Wistar大鼠随机分为正常组(10只)和造模组(38只)。模型组采用复合因素造模法制备肝郁脾虚型FD大鼠模型,持续造模10 d。将造模成功的35只大鼠随机均分为模型组、平胃胶囊低、中、高剂量组和阳性组(莫沙必利),连续给药21 d,每日2次。采用RT-qPCR法、免疫组化SP法、蛋白质免疫印迹法检测各组C型钠尿肽(CNP)、B型钠尿肽受体(NPR-B)、环磷酸鸟苷(cGMP)依赖性蛋白激酶((Prkg1)的表达。结果 与正常组比较,模型组CNP、NPR-B、Prkg1的mRNA表达、平均光密度值及蛋白表达均显著升高,表明造模成功。与模型组比较,平胃胶囊低、中、高剂量组大鼠CNP、NPR-B、Prkg1 mRNA的表达降低,阳性组大鼠CNP、NPR-B mRNA的表达降低。除平胃胶囊高剂量组Prkg1的表达水平降低无统计学意义外,其余各药物干预组CNP、NPR-B、Prkg1的表达显著降低。与模型组大鼠比较,除平胃胶囊低剂量组大鼠CNP蛋白的表达增加外,其余各组CNP、NPR-B、Prkg1蛋白的表达明显降低。结论 肝郁脾虚型FD模型大鼠存在CNP/NPR-B/cGMP信号通路改变;平胃胶囊可能与通过下调该信号通路来促进胃肠动力。
OBJECTIVE To explore the pathogenesis of functional dyspepsia(FD) with liver stagnation and spleen deficiency and the intervention mechanism of Pingwei capsules in promoting gastrointestinal motility.METHODS A total of 48 Wistar male rats were randomly divided into normal group(n=10) and model group(n=38).The FD model of liver stagnation and spleen deficiency was established by compound method for 10 days in the model group.A total of 35 rats in the model group were randomly divided into 5 groups: model group, low-dose, middle-dose and high-dose of Pingwei capsules groups, as well as positive(mosapride) group.The drug was administered twice a day for 21 days.The expressions of CNP,NPR-B and cGMP-dependent protein kinase(Prkg1) in each group were detected by RT-qPCR,immunohistochemical SP method and Western blotting.RESULTS Compared with the normal group, the mRNA expression level, average optical density value, and protein expression of CNP,NPR-B and Prkg1 in the model group were significantly increased.Compared with the model group, the expression of CNP,NPR-B and Prkg1 mRNA was decreased in the low-dose, medium-dose and high-dose Pingwei capsules groups, and the expression of CNP and NPR-B mRNA was decreased in the mosapride group.The expression levels of CNP,NPR-B and Prkg1 in the drug intervention groups were decreased, except that the expression level of Prkg1 in the high-dose Pingwei capsules group had no significant reduction.Compared with the model group, the protein expression of CNP in the low-dose Pingwei capsules group was increased, and the protein expression of CNP,NPR-B and Prkg1 in the other groups was significantly decreased.CONCLUSION There are changes in CNP/NPR-B/cGMP signaling pathway in FD model rats of liver stagnation and spleen deficiency, and Pingwei capsules may promote gastrointestinal motility by down-regulating this signaling pathway.
作者
张萍
毛兰芳
汪龙德
李红芳
吴红莉
王淼蕾
牛媛媛
王静静
范玉春
ZHANG Ping;MAO Lanfang;WANG Longde;LI Hongfang;WU Hongli;WANG Miaolei;NIU Yuanyuan;WANG Jingjing;FAN Yuchun(Gansu University of Traditional Chinese Medicine,Lanzhou,Gansu,730000 P.R.China;Affiliated Hospital of Gansu University of Traditional Chinese Medicine,Lanzhou,Gansu,730200 P.R.China;Lanzhou University,Lanzhou,Gansu,730000 P.R.China)
出处
《华西药学杂志》
CAS
CSCD
2023年第1期47-51,共5页
West China Journal of Pharmaceutical Sciences
基金
国家自然科学基金资助项目(批准号:82160883)
甘肃中医药大学科学研究与创新基金重点项目(30140301)。
关键词
功能性消化不良
肝郁脾虚型
疏肝健脾法
平胃胶囊
胃肠动力
情志致病
C型尿钠肽信号通路
Functional dyspepsia
Liver stagnation and spleen deficiency
Soothing liver and invigorating spleen method
Pingwei capsules
Gastrointestinal motility
Emotional disease
C-type natriuretic peptide signaling pathway
