摘要
目的通过建立白芍药材、饮片、标准汤剂HPLC特征图谱对白芍生产过程的共有峰进行定性分析,结合白芍药材-饮片-标准汤剂质量传递过程筛选白芍质量标志物(Q-Marker)。方法采集10批白芍鲜药材,制备为药材、饮片和标准汤剂,采用HPLC建立白芍药材-饮片-标准汤剂特征图谱,结合相似度评价及正交偏最小二乘-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)等分析初步确定的指标成分在药材-饮片-标准汤剂过程的含量及转移率,探究白芍生产过程中的质量传递规律,综合筛选白芍Q-Marker。结果白芍药材、饮片、标准汤剂特征图谱的共有峰有9个,组内相似度较高,OPLS-DA结果显示,9号共有峰不能稳定传递;白芍药材-饮片传递过程中,没食子酸、氧化芍药苷、儿茶素、芍药内酯苷、芍药苷、没食子酰芍药苷、1,2,3,4,6-O-五没食子酰葡萄糖、苯甲酰芍药苷8种成分转移率大于70%,可以传递稳定;饮片-标准汤剂传递过程中,芍药苷、芍药内酯苷、没食子酰芍药苷、氧化芍药苷、苯甲酰芍药苷等单萜及其苷类成分含量稳定且转移率大于60%,可以传递稳定;没食子酸转移率大于200%,未能充分体现白芍饮片中没食子酸的传递情况;儿茶素和1,2,3,4,6-O-五没食子酰葡萄糖2种成分转移率小于25%。结论结合白芍药材、饮片和标准汤剂中特征图谱、含量及转移率变化等,综合筛选确定氧化芍药苷、芍药内酯苷、芍药苷、没食子酰芍药苷、苯甲酰芍药苷5种成分作为白芍Q-Marker。
Objective To qualitatively analyze the co-peaks in the production process of Baishao(Paeoniae Radix Alba,PRA)by HPLC specific chromatogram established by PRA medicinal materials,decoction pieces and standard decoctions,and screen Q-Marker of PRA combining the quality transfer process of medicinal materials,decoction pieces and standard decoctions.Methods Collecting 10 batches of PRA and preparing them as medicinal materials,decoction pieces and standard decoctions,HPLC was adopted to establish the specific chromatogram of medicinal materials,decoction pieces and standard decoctions of PRA,combined with the similarity evaluation and OPLS-DA,the content and transfer rate of the preliminarily determined indicator components in the process of medicinal materials,decoction pieces and standard decoctions were analyzed,the quality transfer law in the production process of PRA was explored,and the Q-Marker of PRA was comprehensively screened.Results There were nine co-peaks in the HPLC specific chromatogram of decoction pieces and standard decoctions of PRA and the intragroup similarity was high.OPLS-DA results showed that No.9 co-peak cannot be transmitted stably.In the process of medicinal materials-decoction pieces,the transfer rate of gallic acid,oxypaeoniflorin,catechin,albiflorin,paeoniflorin,gallophilyl paeoniflorin,1,2,3,4,6-O-pentagalloglucose,benzoylpaeoniflorin was more than 70%,which can transfer stability.In the process of decoction pieces-standard decoctions,the transfer rate of oxypaeoniflorin,albiflorin,paeoniflorin,gallophilyl paeoniflorin,benzoylpaeoniflorin mainly monoterpenes and their glycosides was stable and the transfer was more than 60%,which can transfer stability;The transfer rate of gallic acid was more than 200%,the transmission of gallic acid in the decoction pieces of PRA was not fully reflected.The contents of catechin,1,2,3,4,6-Opentagalloglucose were unstable and the transfer rate was less than 25%.Conclusion Combined with the HPLC specific chromatogram,content and transfer rate of compo
作者
岳倩侠
孙叶芬
杨爽
杜倩倩
金传山
张伟
程世云
朱月健
王翔
YUE Qian-xia;SUN Ye-fen;YANG Shuang;DU Qian-qian;JIN Chuan-shan;ZHANG Wei;CHENGShi-yun;ZHU Yue-jian;WANG Xiang(Anhui University of Chinese Medicine,Hefei 230012,China;Anhui Province Key Laboratory of Traditional Chinese Medicine Decoction Pieces of New Manufacturing Technology,Hefei 230012,China;Anhui Institute for Food and Drug Control,Hefei 230051,China;Anhui Jiren Pharmaceutical Co.,Ltd.,Bozhou 236800,China)
出处
《中草药》
CAS
CSCD
北大核心
2023年第2期553-560,共8页
Chinese Traditional and Herbal Drugs
基金
国家重点研发计划(2019YFC1711505)
安徽省重点研发项目(202104h04020029)
亳州市科技重大项目专项(bzzd2020002)。
