摘要
为解析芝麻耐铝毒遗传机制,发掘和定位芝麻耐铝毒QTL,采用不同浓度铝离子对地方品种金黄麻和竹山白进行了芽期胁迫处理,确定芽期铝胁迫处理适宜浓度。利用耐铝毒芝麻种质金黄麻和铝敏感芝麻种质竹山白为亲本构建的一个含有180个家系的重组自交系群体,以铝胁迫下芝麻芽期相对根长(RRL)、相对芽长(RSL)和相对苗鲜质量(RSW)作为表型指标,结合通过重测序构建的一个含有1354个bin标记的高密度遗传图谱,采用复合区间作图法,对芝麻芽期耐铝毒性状进行了QTL定位和候选基因筛选。3个性状在群体中呈显著正相关,共检测到7个QTL,分布于6条染色体上(2,4,6,10,11,13号染色体),其中3个与相对根长相关,2个与相对芽长相关,2个与相对苗鲜质量有关。其中位于2号染色体的2个分别控制相对根长和相对苗鲜质量的QTL区间部分重叠。候选基因分析的结果表明,15个预测基因可能与耐铝胁迫相关,主要参与金属转运、脱毒和金属离子的跨膜结合、GDSL基序酯酶、过氧化物酶体以及生物体内有毒物质的代谢与解毒等生理进程。
To elucidate the genetic mechanism and identify quantitative trait loci(QTL)for Aluminum(Al)tolerance in sesame.We investigated the response of Al stress using sesame landraces Jinhuangma(JHM)and Zhushanbai(ZSB)with different concentrations of Al 3+and confirmed a suitable Al 3+concentration for stress.Three traits including relative root length(RRL),relative shoot length(RSL)and relative fresh seedling weight(RSW)were evaluated in a recombinant inbred line(RIL)population with 180 lines that derived from two parents that contrasts for Al tolerance(ZSB and JHM).Quantitative trait loci(QTL)mapping were conducted by using a high resolution genetic map containing 1354 bin markers through composition interval mapping(CIM)method.Significant positive correlations were observed among the three traits.A total of 7 QTLs were detected on 6 chromosomes(Chr2,Chr4,Chr6,Chr10,Chr11 and Chr13),including 3 RRL related QTLs,2 RSL related QTLs and 2 RSW associated QTLs.Among them,two QTLs associated with RRL and RSW were mapped to the overlapped interval on chromosome 2.Putative candidate gene analysis revealed that 15 genes may be related to Al tolerance.These genes mainly involved in metal transport and detoxification,transmembrane binding of metal ions,GDSL-motif lipase,peroxisomal catalase and metabolism and detoxification of toxic substances in organisms.
作者
梁俊超
赵云燕
王郅琪
孙建
颜廷献
颜小文
饶月亮
周红英
乐美旺
LIANG Junchao;ZHAO Yunyan;WANG Zhiqi;SUN Jian;YAN Tingxian;YAN Xiaowen;RAO Yueliang;ZHOU Hongying;LE Meiwang(Crops Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;Nanchang Branch of National Center of Oil Crops Improvement,Nanchang 330200,China;Jiangxi Province Key Laboratory of Oil Crops Biology,Nanchang 330200,China;College of Agriculture,Yangtze University,Jingzhou 330033,China)
出处
《华北农学报》
CSCD
北大核心
2022年第6期124-131,共8页
Acta Agriculturae Boreali-Sinica
基金
江西省青年科学基金项目(20202BABL215005)
国家自然科学基金(32160482)
国家特色油料产业技术体系项目(CARS-14)。
