摘要
MYB(v-myb avian myeloblastosis viral oncogene homolog)是调控植物木质素合成的转录因子之一。为探讨绿萼凤仙花木质素合成调控的分子机制,本试验克隆了绿萼凤仙花MYB4基因,对其进行生物信息学分析,并利用qRT-PCR技术分析其表达模式。结果表明:(1)成功克隆到绿萼凤仙花MYB4基因的3个片段,其cDNA全长分别为666、888、771 bp,编码221、295、256 aa,命名为IcMYB4-1、IcMYB4-2和IcMYB4-3。(2)3个IcMYB4基因都含有MYB家族的DNA结合结构域(R2和R3)以及抑制型的保守基序EAR,其氨基酸序列与其他物种具有较高的相似性,同源性达62%左右。IcMYB4-1和IcMYB4-2氨基酸处于一个分支,推测其为直系同源关系;IcMYB4-3与前两者分属两个分支,推测其为旁系同源关系。(3)qRT-PCR结果表明,3个IcMYB4基因在绿萼凤仙花的不同部位均有表达,其中IcMYB4-1在绿萼凤仙花不同部位的表达均为最低,以根部表达量最高;IcMYB4-2和IcMYB4-3基因在绿萼凤仙花的根、茎和叶中的表达量依次升高,推测其对绿萼凤仙花木质素合成起负调控作用。综上所述,IcMYB4基因在绿萼凤仙花不同部位的作用机理存在一定差异,但均在绿萼凤仙花的木质素合成调控中发挥了重要作用。该结果为增加凤仙花的木质化程度以及品种改良提供了基础数据。
MYB(v-myb avian myeloblastosis viral oncogene homolog) is one of the transcription factors regulating plant lignin synthesis. In order to explore the molecular mechanism of lignin synthesis regulation in Impatiens chlorosepala, the MYB4 genes of I. chlorosepala were cloned and analyzed by bioinformatics, and its expression patterns were investigated by qRT-PCR method. The results were as follows.(1)Three MYB4 genes of I. chlorosepala were successfully cloned, whose full-length cDNA were 666, 888 and 771 bp, encoding 221, 295 and 256 aa, and named IcMYB4-1, IcMYB4-2 and IcMYB4-3,respectively.(2) All the threee IcMYB4 genes contained the DNA-binding domains(R2 and R3) of MYB family and the suppressive conservative EAR motifs. The amino acid sequence of MYB4 genes of I. chlorosepala had high similarity with that of other species, and the homology was about 62%. IcMYB4-1 and IcMYB4-2 amino acids were clustered into one branch, which inferred that they had lineal homology;IcMYB4-3 was separated from IcMYB4-1 and IcMYB4-2, which inferred that they had paraphyletic homology.(3) Based on the results of qRT-PCR, it showed that the three IcMYB4 genes were expressed in different parts of I. chlorosepala, among which, the expression level of IcMYB4-1 was the lowest in different parts, while that in roots was the highest. The expression level of IcMYB4-2 and IcMYB4-3 genes in the roots, stems and leaves increased in order, which was speculated that both IcMYB4-2 and IcMYB4-3 had negative regulation effects on lignin synthesis of I. chlorosepala. In conclusion, the action mechanism of IcMYB4 genes in different parts of I. chlorosepala was different, but all of them played an important role in the regulation of lignin synthesis. The results provided basic data for increasing the degree of lignification and variety improvement of Impatiens.
作者
李泽凤
向南星
魏春梅
李新艺
孟丹晨
黄海泉
黄美娟
Li Zefeng;Xiang Nanxing;Wei Chunmei;Li Xinyi;Meng Danchen;Huang Haiquan;Huang Meijuan(College of Landscape and Horticulture,Southwest Forestry University/Southwest Research Center for Engineering Technology of Landscape Architecture,State Forestry and Grassland Administration/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization/Research and Development Center of Landscape Plants and Horticulture Flowers,Southwest Forestry University,Kunming 650224,China)
出处
《山东农业科学》
北大核心
2023年第1期8-14,共7页
Shandong Agricultural Sciences
基金
国家自然科学基金项目(32060364,32060366,31860230,31560228)
云南省重大科技专项(202102AE090052)
云南省高校园林植物与观赏园艺科技创新团队项目(51700204)
云南省园林植物遗传改良与高效繁育博士生导师团队和云南省中青年学术和技术带头人培养项目(2018HB024)。
