摘要
目的:探讨一种基于褪黑素(MT)的分子探针对胶质母细胞瘤的成像效果。方法:以5-甲氧基色胺(褪黑素)为原料,将其与荧光染料Cy5偶联制得探针Cy5-MT。采用MTT法测试探针Cy5-MT对胶质母细胞瘤细胞U87和正常的人脑胶质细胞HEB的细胞毒性;采用流式细胞实验测试探针Cy5-MT对胶质母细胞瘤细胞U87的靶向性;采用激光共聚焦实验测试探针Cy5-MT对U87的成像效果。结果:探针Cy5-MT的结构通过质谱进行确证。Cy5-MT对U87和HEB的毒性较小,Cy5-MT在U87细胞中的百分摄取率显著高于HEB细胞;Cy5-MT能够特异性地对U87细胞进行成像。结论:探针Cy5-MT能够特异性的靶向胶质母细胞瘤U87细胞,实现对U87的精准成像,且生物安全性高。
Objective: To explore the imaging effect of a melatonin-based molecular probe in glioblastoma.Methods: The probe Cy5-MT was prepared by coupling 5-methoxytryptamine with fluorescent dye Cy5. The in vitro MTT assay was used to evaluate the toxic effect of Cy5-MT on glioblastoma cell U87 and normal human glial cell HEB;Flow cytometry was used to evaluate the targeting ability of Cy5-MT to U87 and HEB;laser scanning confocal microscopy was used to evaluate the imaging effect of Cy5-MT on U87. Results: The structure of Cy5-MT was confirmed by mass spectrometry. In vitro cytotoxicity study showed that Cy5-MT had low toxicity to U87 and HEB, indicating high biosafety. Flow cytometry results showed that the percentage of cellular uptake and mean fluorescence intensity of the probe per U87 cells were significantly higher than the normal HEB cells.Imaging results showed that Cy5-MT can accurately image U87 tumor cells. Conclusion: The probe Cy5-MT can specifically target U87 glioblastoma cells to achieve accurate imaging of U87 and also demonstrates high biosafety.
作者
万家颖
王银
WAN Jia-ying;WANG Yin(Department of Nephrology,Wuhan NO.1 Hospital(Wuhan Hospital Of Traditional Chinese AndWestern Medicine),Wuhan 710003,China;Department of Clinical Laboratory,Wuhan NO.1 Hospital(Wuhan Hospital Of Traditional Chinese AndWestern Medicine),Wuhan 710003,China)
出处
《神经损伤与功能重建》
2023年第2期77-79,共3页
Neural Injury and Functional Reconstruction
